中文摘要：

目的：探讨下调miR-181a对人胃癌细胞生物学行为的影响。方法：转染miR-181ainhibitor下调miR-181a在人胃癌细胞SGC-7901中的表达。通过MTT法、流式细胞仪、Annexin-V＋PI双染色法和Tr-answell实验,观察下调miR-181a表达对人胃癌细胞增殖、凋亡及侵袭迁移等生物学行为的影响。结果：瞬时转染miR-181ainhibitor后胃癌细胞SGC-7901中miR-181a的表达明显下调（P=0.0027）。转染miR-181ainhibitor后7901细胞增殖明显减弱（P=0.0006）,被阻滞的G0/G1期细胞明显增多（P=0.0075）,S期细胞明显减少（P=0.0022）,细胞凋亡比例增加（P〈0.0017）。Transwell实验证实下调miR-181a表达能促进人胃癌细胞侵袭与迁移（P=0.0006、P=0.0004）。结论：下调miR-181a表达抑制胃癌细胞增殖、促进凋亡,促进侵袭迁移,提示miR-181在胃癌的发生发展中可能发挥重要的作用。

英文摘要：

Objective：To investigate the influence of down -regulating miR - 181a on biological behavior of gastric cancer cell. Methods： miR- 181a inhibitor was transfected transiently into gastric cancer cell SGC -7901 to down - regulate the expression of miR - 181a. The influence of down - regulation of miR - 181a on cell proliferation, apoptosis,invasion and migration were evaluated by MTT,flow cytometry,Annexin -V ＋ PI and transwell assay. Results： The transfection of miR- 181a inhibitor down- regulated miR- 181a expression in SGC -7901 （P = 0. 002 7）. In SGC -7901 cell after transfection with miR- 181a inhibitor, the proliferative ability was inhibited （P = 0. 000 6）, G0/G1 phase cell ratio was arrested （P = 0. 007 5）, S phase cell ratio was decreased （P = 0. 002 2）, and apoptosis rate was increased （P 〈 0. 001 7）. Transwell assay showed cell invasion and migration was increased greatly in miR - 181 a down - regulated SGC - 7901 cell （ P = 0. 000 6, P = 0. 000 4 ）. Conclusion ： Down - regulation of miR - 181 a inhibits gastric cancer cell proliferation and promotes apoptosis, but enhances cell invasion and migration. It may play an important role in gastric carcinogenesis.