中文摘要：

目的：应用荧光染料SYBR-14／碘化丙锭（PI）双标法流式细胞术检测溶脲脲原体（Uu）感染对男性不育患者精子质膜完整性的影响。方法：收集63例男性精液标本，分为uu感染组（n=32）和正常对照组（n=31）。通过计算机辅助精液分析系统进行精液常规分析，培养法进行Uu检测。精液标本经PBS洗涤处理后用荧光染料SYBR-14／PI双染后上流式细胞仪分析，用发绿色荧光精子百分率（SYBR-14＋／PI-％）表示质膜完整精子的比例，检测精子质膜完整性。结果：uu感染组精子质膜完整性[（45．14±10．69）％versus（72．68±9．91）％]和（a＋b）级精子百分率[（23．29±8．81）％versus（46．32±9．54）％]均显著低于正常对照组（P〈0．01），两组问精液量、pH值、精子浓度差异无显著性（P〉0．05）。结论：uu感染引起精子质膜完整性下降，可能是导致男性不育的重要原因之一。

英文摘要：

Objective： To determine the impact of Ureaplasma urealyticum （Uu） infection on the integrity of sperm plasma membrane in infertile males. Methods： Sixty-three semen samples were divided into a Uu infection group （n = 32 ） and a normal control group （n = 31 ）. Conventional semen analyses were performed by computer-assisted semen analysis （CASA） and Uu detected by the culture method. The semen samples were washed with PBS and dyed by SYBR-14/PI double fluorescent staining, followed by detection of the integrity of sperm plasma membrane by flow cytometry. The percentage of the sperm with intact plasma membrane was indicated as the percentage of sperm emitting green fluorescence （ SYBR-14 ＋/PI - % ）. Results ： The Uu infection group showed a significantly decreased integrity of sperm plasma membrane （ [ 45. 14± 10.69 % ） and reduced percentage of grade a ＋ b sperm （[23.29±8.811%） as compared with the normal control group （[72.68±9.91] % and [46.32±9.54] %） （P〈0.01）. But there were no significant differences in the semen volume, pH value, and sperm concentration between the two groups （P 〉 0.05 ）. Conclusion ： Uu infection decreases the integrity of sperm plasma membrane, which might be an important factor of male infertility.