中文摘要：

目的：研究骨髓间充质干细胞（BM-MSC）对MRL／lpr狼疮鼠B淋巴细胞活化及AKT／GSK3信号通路的影响。方法：从C57／BL6小鼠骨髓细胞中分离培养BM-MSC，用不同剂量的BM-MSC移植治疗MRL／lpr狼疮鼠，4周后免疫磁珠分选出B淋巴细胞，流式细胞仪检测细胞周期，Western Blot检测AKT／GSK3β信号通路相关蛋白表达变化。结果：狼疮鼠B淋巴细胞体外刺激后s期比例明显增多，中、高剂量BM-MSC移植治疗能降低刺激后的狼疮鼠B淋巴细胞s期比例，并增加细胞周期蛋白p27^Kip1的表达。BM-MSC移植治疗能抑制B淋巴细胞phospho—AKT^Thr308及phospho-GSK3β^Ser9。的表达。结论：中、高剂量BM—MSC移植治疗能抑制狼疮鼠B淋巴细胞的异常活化，并抑制AKT／GSK3β信号通路的异常活化。

英文摘要：

Objective：To investigate the effect of BM-MSC in regulating the cell cycle and the expression of AKT/GSK3β signal pathway of B lymphocytes from MRL/lpr lupus mice. Methods： BM-MSC were separated from C57/BL6 mice and cultured for the following experiments. Lupus mice were divided into 4 groups receiying BM-MSC transplantation in different concentrations re- spectively. Four weeks after transplantation, B lymphocytes were isolated by magnetic activated cell sorting. Cell cycles were detected by FACScan flow cytometer. The expressions of AKT/ GSK3β signal pathway were measured by Western-blotting. Results：The population of S phase of activated B lymphocytes from lupus mice increased compared to control group. Activated B lym- phocytes from lupus mice receiving middle or high dose BM-MSC transplantation were accumulated at G0/G1 checkpoint. The expressions of phospho-AKT^Thr308 and phospho-GSK3β^ser9 decreased while p27^Kip1 increased in the groups treated with BM-MSC. Conclusion：Middle or high-dose BM- MSC treatment had an inhibitory effect on G1/S transition of the abnormal lupus B lym-phocytes. BM-MSC could also inhibit abnormal activation of the AKT/GSK3β signaling pathway of B cellsfrom MRL/lpr mice.