中文摘要：

目的 观察佐剂性关节炎（AA）大鼠滑膜中自噬相关基因5、7、12（Atg5,7,12）mRNA、微管相关蛋白1轻链3-Ⅱ（LC3-Ⅱ）、自噬标志物Beclin1、磷脂酰肌醇3-激酶（PI3K）、蛋白激酶B（AKT）、哺乳动物雷帕霉素靶向蛋白（m Tor）及血清细胞因子IL-1β、TNF-α、IL-4、IL-10的表达水平变化及新风胶囊对其的影响。方法将48只雄性SD大鼠随机分为对照组、模型组、西药组（来氟米特,5.0 mg/kg）和中药组（新风胶囊,3.0 g/kg）,每组12只。干预30天后,观察各组大鼠体质量、足趾肿胀度（E%）、关节炎指数（AI）及踝关节病理及超微结构变化;检测大鼠滑膜中Atg5、7、12 mRNA表达;检测滑膜LC3-Ⅱ、Beclin1、PI3K、AKT、m Tor蛋白表达;检测大鼠血清IL-1β、TNF-α、IL-4、IL-10含量。结果 与正常组比较,模型组E%、AI、IL-1β、TNF-α升高,体重、IL-4、IL-10、Atg5 mRNA、Atg12 mRNA、LC3-Ⅱ、Beclin1蛋白表达降低（P〈0.01,P〈0.05）,PI3K、AKT、m Tor蛋白表达升高（P〈0.01）。与模型组比较,两个给药组E%、AI、IL-1β、TNF-α、Atg12 mRNA、PI3K、AKT、m Tor蛋白表达降低（P〈0.01,P〈0.05）,IL-4、IL-10、LC3-Ⅱ、Beclin1蛋白表达升高（P〈0.01,P〈0.05）;西药组Atg5降低（P〈0.01）,Atg7 mRNA升高（P〈0.05）。与西药组比较,中药组体重、IL-4、Atg5 mRNA、Atg12 mRNA、PI3K、m Tor蛋白表达升高（P〈0.01,P〈0.05）。结论AA大鼠滑膜细胞自噬水平下降,导致滑膜细胞的过度增生,引起关节肿胀,致炎因子上升,抑炎因子下降,引起关节的炎症反应。中药新风胶囊可以改善关节炎指数、足趾肿胀度,改善滑膜自噬相关基因及蛋白表达。

英文摘要：

Objective To observe expression levels of autophagy related 5,7,12 mRNA （Atg5, 7,12）, microtubule-associated protein 1 light chain 3 （LC3-Ⅱ）, Beclinl, phosphatidylinositol 3-kinase （PI3K）, protein kinase B （AKT）, mammalian target of rapamycin （mTOR）, IL-1β, TNF-α, IL-4, and IL- 10 in adjuvant arthritis （AA） rats, and effects of Xinfeng Capsule （XFC） on them. Methods Totally 48 male SD rats were divided into 4 groups, i.e., the control group, the model group, the Western medicine （WM） group （leflunomide, 5.0 mg/kg）, the Chinese medicine （CM） group （Xinfeng Capsule, 3.0 g/kg）, 12 in each group. Thirty days after medication body weight （BW）, toe swelling degree （E%）, arthritis in- dex （AI）, and pathological changes of ankle joint and ultrastructural changes were observed, mRNA ex- pressions of Atg5,7, 12, protein expressions of LC3-Ⅱ, Beclinl, PI3K, AKT, mTOR, serum contents of IL-1 β, TNF-α, IL-4, and IL-10 were detected. Results Compared with the normal group, E%, AI, IL-1β and TNF-α increased; BW, levels of IL-4 and IL-10, mRNA expressions of Atg5 and Atg12, protein ex-pressions of LC3-Ⅱ and Beclinl decreased （P 〈0.01, P 〈0.05）, protein expressions of PI3K, AKT, mTOR increased （P 〈0.01 ） in the model group. Compared with the model group, E%, AI, mRNA expres- sions of IL-1β, TNF-α, and Atg12, protein expressions of PI3K, AKT, and mTOR decreased （P 〈0.01, P〈0.05）, IL-4, IL-10, protein expressions of LC3-Ⅱ and Beclinl increased （P 〈0.01, P 〈0.05） in the two medicated groups. Atg5 mRNA expression decreased （P 〈0.01 ）, Atg7 mRNA expression increased （P 〈 0.05） in the WM group. Compared with the WM group,BW, IL-4, mRNA expressions of Atg5 and Atg12, protein expressions of PI3K and mTOR increased in the CM group （P 〈 0.01, P 〈 0.05）. Conclusions The level of autophagy in AA rats was decreased, leading to excessive proliferation of synovial cells, swollen joints, elevated proinflammatory factors, decr