决定微分基因在是有尖锐心肌的梗塞(AMI ) 的 Wistar 老鼠的化学家心肌层,我们构造了二微分基因表示侧面。AMI 模型被左前面的下降冠的动脉的结扎在 Wistar 老鼠产生。全部的 RNA 从正常被提取并且是在在在操作以后的第 8 天的结扎点下面的化学家心纸巾。二件样品的微分基因表示侧面被使用基因表示(LongSAGE ) 的长连续的分析构造。实时荧光量的 PCR (Q-PCR ) 被用来证实部分目标基因的表示变化。主要结果如下:15966 个标签的一个总数从正常被屏蔽并且在正常织物和 9563 个标签是化学家 LongSAGE 地图,和 9646 个标签在是化学家织物被获得。在他们之中, 7665 个新奇标签被 NCBI 强风搜索识别。在里面是化学家织物, 142 基因显著地在正常织物与那些相比变化了(P 【 0.05 ) 。表示基因可以玩的这些差别在氧化和磷酸化作用, ATP 合成和醣原酵解的小径的重要角色等等。LongSAGE 识别的部分基因被 Q-PCR 证实。结果证明 AMI 在与能量代谢有关的小径的规定引起一系列基因表示变化。
To determine the differential genes in ischemic myocardium of Wistar rats with acute myocardial infarction (AMI), we constructed two differential gene expression profiles. AMI model was generated by Iigation of the left anterior descending coronary artery in Wistar rats. Total RNA was extracted from the normal and the ischemic heart tissues under the IigaUon point at the 8th day after the operation. Differential gene expression profiles of the two samples were constructed by using long serial analysis of gene expression (LongSAGE). Real time fluorescence quantitative PCR (Q-PCR) was used to confirm the expression changes of partial target genes. The main results were as follows: a total of 15966 tags were screened from the normal and the ischemic LongSAGE maps, and 9646 tags in the normal tissue and 9563 tags in the ischemic tissue were obtained. Among them, 7665 novel tags were identified by NCBI BLAST search. In the ischemic tissue, 142 genes significantly changed compared to those in the normal tissue (P〈0.05). These differentially expressed genes may play important roles in the pathways of oxidation and phosphorylation, ATP synthesis and glycolysis and so on. Partial genes identified by the LongSAGE were confirmed by Q-PCR. The results show that AMI causes a series of gene expression changes in the regulation of the pathways related to energy metabolism.