荒芜的湖,在荒芜的生态系统的唯一的绿洲,港口不同细菌的社区。因此,它被看作细菌的差异的一个中心。在这研究,在弦月春天的沉积的细菌的差异, Kumtag 沙漠,向西北,中国用高产量的 amplicon pyrosequencing 分析被分析。在弦月春天的沉积的最丰富的 OUTs (运作的分类单位) 的序列与那些大多数的序列相比从 NCBI GenBank 的丰富的 OUTs of 各种各样的起源在弦月春天的沉积检测细菌的起源的数据库。另外,在春天和其它抛弃的弦月的沉积之间的细菌的作文和湖生态系统(包括的荒芜的湖) 全球用簇分析被比较决定影响细菌的作文的可能的因素。总共, 11,855 个序列被获得, 30 数被识别。在门水平,主导的门是有是的 -Proteobacteria 的 Proteobacteria 第一个主导的班和第二扇主导的门是 Planctomycetes。我们发现那 -Proteobacteria 是是的 Proteobacteria 和 Planctomycetes 的第一个主导的班第二个主导的数与从另外的沙漠的报告是有点矛盾的湖沉积。这差别能从水水和和电导率被结果,以及弦月的寡营养的条件跳。在类水平, Rhodobacter, Caldilinea, Planctomyces,和 Porphyrobacter 是在弦月春天的沉积的主导的类。最丰富的 OUTs 的序列上的比较(包括 OTU3615, OTU6535,和 OTU6646 ) 在从 NCBI GenBank 的弦月春天和各种各样的起源的沉积之间数据库显示在弦月春天的沉积的细菌的起源从地下的水是可能的。而且,聚类分析上在弦月春天和其它沙漠和湖生态系统(包括的荒芜的湖) 的沉积之间的细菌作文的比较全球在地区性的规模显示出那,细菌的作文可以是主要由地理模式,降水数量,和 pH 价值影响了。一起,我们的结果在荒芜的湖生态系统在细菌的差异上提供新知识。
Desert lake, a unique oasis in desert ecosystems, harbours different bacterial communities. Thus, it is considered as a hub of bacterial diversity. In this study, bacterial diversity in the sediment of Crescent Moon Spring, Kumtag Desert, Northwest China was analyzed using high-throughput amplicon pyrosequencing analysis. The sequences of the most abundant OUTs (Operational Taxonomic Units) in the sediment of Crescent Moon Spring were compared with the sequences of those most abundant OUTs of various origins from NCBI GenBank database to detect the origins of bacteria in the sediment of Crescent Moon Spring. Also, bacterial compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (including desert lakes) worldwide were compared using cluster analysis to determine the possible factors affecting bacterial compositions. In total, 11,855 sequences were obtained and 30 phyla were identified. At the phylum level, the dominant phylum was Proteobacteria with α-Proteobacteria being the first dominant class and the second dominant phylum was Planctomycetes. Our finding that α-Proteobacteria being the first dominant class of Proteobacteria and Planctomycetes being the second dominant phyla are somewhat contradictory with reports from other desert lake sediments. This difference could be resulted from water hydration and conductivity, as well as oligotrophic conditions of Crescent Moon Spring. At the genus level, Rhodobacter, Caldilinea, Planctomyces, and Porphyrobacterwere the dominant genera in the sediment of Crescent Moon Spring. Comparisons on sequences of the most abundant OUTs (including OTU3615, OTU6535, and OTU6646) between sediment of Crescent Moon Spring and various origins from NCBI GenBank database indicate that the origins of bacteria in the sediment of Crescent Moon Spring are likely from the underground water. Furthermore, cluster analysis on comparisons of bacteria compositions between sediment of Crescent Moon Spring and other desert and lake ecosystems (inc