中文摘要：

目的探讨干扰素（IFN）-α、吉非替尼对人结肠癌细胞系HCTll6的增殖、凋亡作用。方法采用结肠癌细胞HCTll6为研究对象，观察不同浓度IFN—α（50U／ml和100U／ml）、吉非替尼（0．5μmol／L，2．5μmol／L和5．0μmol／L）单独和联合应用（IFN—α50U／ml＋吉非替尼0．5μmol／L）和不同作用时间点（作用24、48和72h）对细胞的生物学作用。四甲基偶氮唑盐（MTT）法检测药物对细胞的增殖抑制作用，光学显微镜观察细胞形态变化，流式细胞学检测细胞凋亡情况。采用SPSS13．0软件进行统计学分析，两组间比较采用t检验，多组数据比较采用单因素方差分析。结果IFN—α、吉非替尼单独和联合应用均能明显抑制HCT116的增殖（P值均〈0．05），且抑制程度与药物作用时间和药物浓度之间存在依赖效应。药物作用下可观察到细胞呈现凋亡形态学改变，流式细胞学结果显示HCT116细胞凋亡率明显增加，IFN—α（50U／ml）、吉非替尼（0．5μmol／L）单独和联合应用72h后细胞凋亡率分别为15．6％±0．6％、13．6％±0．4％和31．2％±0．3％明显高于对照组的6．8％±0．3％，差异均有统计学意义（P值均〈0．01）。结论IFN—α、吉非替尼均能抑制HCT116细胞生长并诱导细胞凋亡，两者联合应用具有协同增效作用。

英文摘要：

Objective To investigate the effects of interferon-α （IFN-α） and gefitinib on the proliferation and apoptosis of human colon cancer cell line HCT116. Methods Colon cancer cell line HCT116 was selected as research objective. The biological effects of IFN-α and gefitinib alone or combined on the cells were observed at different time point （after worked for 24, 48 and 72 hours）. The proliferation inhibition of the medicine on the HCTll6 cells was measured by methyl thiazolyl tetrazolium （MTT） assay. Morphologic changes were observed under optical microscope. Apoptosis was measured by flow cytometry （FCM）. The results were analyzed with SPSS 13.0 software, two groups compare was tested by t test, and single factor variance analysis was for multiple group data compare. Results IFN-α and gefitinib alone or combined could significantly inhibit the proliferation of HCTll6 ceils （P〈0.05）, and there was a time and dose-dependent manner between the degree of inhibition and the working time and concentration of the medicine. With the work of the medicine, apoptosis morphologic changes were observed in the cells. And FCM result indicated that the apoptosis rate significantly increased. After treated with IFN-α and gefitinib alone or combined for 72h, the cell apoptosis rate were 15. 6%±0. 6%, 13. 6%± 0. 4% and 31. 2%± 0. 3% respectively, which was obviously higher than control group （6.8 %±0.3 %, P〈0.05）. Conclusion Both IFN-α and gefitinib were able to inhibit the proliferation and induce apoptosis of HCT116 ceils moreover, and a synergistic effect was observed while combine used there two medicines.