【目的】对从酸马奶中分离出来的10株乳酸菌进行鉴定和生理生化特性研究,为工业生产筛选特性优良的菌种。【方法】通过形态学观察、生理生化特性、分子生物学特性及其对致病菌抑制作用的研究对其进行鉴定,并筛选特性优良菌株。【结果】10株乳酸菌分别为2株Lactobacillus plantarum、2株Enterococcus villorum、2株Enterococcus dispar、3株Enterococcus durans和1株Enterococcus raffinosus;其对Staphylococcus aureus、Escherichia coli和Enteritidis bacillus有不同程度的抑制作用。【结论】菌株HZ24、HZ25具有良好的生物学特性和益生功能,可以应用到食品发酵工业生产中。
[Objective] The aim of the present study was to identify the genera and screen po- tential characteristics bacteria in the lactic acid bacteria isolated from koumiss. ]Methods[ The characteristics of lactic acid bacteria were studied by the experiments on some aspects, such as morphology, physiological and biochemical characteristics, molecular biology and the inhibition of pathogenic bacteria. [Results] There were two strains of Lactobacillus plantarum, two strains of Enterococcus villorum, two strains of Enterococcus dispar, three strains of Enterococcus durans and one strain of Enterococeus raffinosus. The tested bacteria had different degrees of inhibition to Staphylococcus aureus, Escherichia coli and Enteritidis bacillus. [Conclusion] HZ24 and HZ25 were good potential probiotics which may make great contributions to food fermentation industry.