中文摘要：

利用植原体通用引物R16mF2／R16mR1和rp（Ⅱ）F1／rp（Ⅱ）R1对海南木豆丛枝病植原体16SrDNA和部分核糖体蛋白（ribosomal protein，rp）基因序列进行PCR扩增、克隆和测序。获得海南木豆丛枝病植原体16S rDNA基因片段为1430bp，rp基因片段为1170bp。核苷酸同源性比较和系统进化树构建表明，引起海南木豆丛枝病的植原体应属于16SrⅡ组中的亚组iii。本研究首次从分子水平确定了引起我国海南木豆丛枝病的病原物为植原体，明确了其分类地位，为该病害流行学研究和防治提供了理论依据。

英文摘要：

Primers R16mP2/R16mR1 and rp （ Ⅱ ） F1/rp （ Ⅱ ） R1 were used to amplify phytoplasma DNA from infected pigeon pea samples, then the amplified fragments were cloned and sequenced. The 16S rDNA （1 430 bp） and rp gene （1 170 bp） were amplified from infected pigeon pea. Homology analysis and phylogenetic tree showed that Hainan pigeon pea witches ＇ -broom phytoplasma belonged to the 16S r Ⅱ group, iii subgroup. Pigeon pea witches＇-broom phytoplasma was firstly identified by molecular biotechnology and confirmed its taxonomic status in Hainan, it provided a theoretical basis for research on the epidemiology and control of the disease.