中文摘要：

锯凤蝶类与凤蝶科其他类群的系统发生关系及其分类学地位一直存在争议。本研究采用PCR和long PCR技术测定了属于锯凤蝶类的丝带凤蝶Sericinus montelus线粒体基因组全序列;结合已有的其他凤蝶科物种的相应序列数据,基于13个蛋白质编码基因重建了凤蝶科主要类群的系统发生树,探讨了它们之间的系统发生关系。基因组分析结果表明：丝带凤蝶线粒体基因组全长15242bp,包括13个编码蛋白基因（ATP6,ATP8,COⅠ-Ⅲ,ND1-6,ND4L和Cytb）、22个tRNA基因、16S和12SrRNA基因以及非编码的控制区;基因组A,T,G和C含量分别为40.1%,40.8%,7.4%和11.7%,表现出明显的AT偏倚。所有的蛋白质编码基因都使用标准的起始密码子（ATN）;除ND4和ND4L基因使用单个的T作为终止密码子外,其余蛋白编码基因都使用了标准的终止密码子（TAA）。除丝氨酸tRNA的二氢尿苷突环缺失外,所有tRNA基因都形成典型的三叶草型结构。基因组中共存在12个大小介于2～65bp之间的基因间隔区以及15个大小介于1～8bp之间的基因重叠区,其中,存在于COⅡ和tRNALys之间的24bp的间隔区在其他鳞翅目昆虫中未曾见到。以邻接法和最大简约法并基于13个蛋白质编码基因序列对凤蝶科进行了系统发生分析。结果显示,丝带凤蝶和中华虎凤蝶Luehdorfia chinensis先构成一个支系,再和冰清绢蝶Parnassius bremeri构成姊妹群;表明锯凤蝶类应作为族级分类单元归于凤蝶科下的绢蝶亚科。

英文摘要：

The phylogenetic relationship between the zerynthiids and the other groups of Papilionidae has long been a controversial issue.In this study,the complete nucleotide sequences of the mitochondrial genome（mitogenome） of Sericinus montela belonging to the zerynthiids was determined using long PCR and conserved primer walking approaches.Meanwhile,the phylogenetic analysis of this species with other representative papilionid species was conducted to clarify their phylogenetic relationships on the mitogenomic level.The results showed that the entire mitochondrial DNA（mtDNA） molecule is 15 242 bp in length with the content of A,T,G and C of 40.1%,40.8%,7.4% and 11.7%,respectively,and its nucleotide composition of the genome is highly A＋T biased（80.8%）;all the 13 protein coding genes（PCGs） use standard initiation codons ATN,and all the PCGs use common stop codon（TAA）,except for the ND4 and ND4L genes,which terminate with a single T;all tRNA genes form a typical clover-leaf secondary structure,except for the tRNASer（AGN）,whose DHU arm forms a simple loop;there are twelve intergenic spacer regions ranging from 2 to 65 bp in size,and fifteen overlaps ranging from 1 to 8 bp in size in the mitogenome sequence.The neighbor joining and maximum parsimony phylogenetic analyses based on the 13 PCG sequences showed that Sericins montela and Luehdorfia chinensis form a clade that is sister to the Parnassius bremeri,suggesting that they should be designated as a taxon of tribal level within the subfamily Parnassiinae in the family of Papilionidae.