以7个蔗茅野生种及其与甘蔗杂交的3个后代材料[滇蔗品种(系)]为试验材料,在苗期进行低温(3℃)胁迫,测定与耐寒性相关的各项生理生化指标。结果表明:(1)通过极点排序法计算得到电导率,可溶性糖、丙二醛、脯氨酸、可溶性蛋白、叶绿素含量,超氧化物歧化酶活性的权重系数分别为0.109、0.194、0.148、0.133、0.108、0.183、0.124,其中可溶性糖与叶绿素含量的权重系数最大,说明这两项生理指标对甘蔗耐寒性影响较大;(2)在低温(3℃)胁迫下,10个供试材料的耐寒性强弱为蔗茅99-3〉蔗茅99-2〉蔗茅Ⅱ91-2〉蔗茅99-4〉蔗茅99-1〉蔗茅90-29〉蔗茅Ⅰ91-8〉滇蔗04-14〉滇蔗01-58〉滇蔗02-39,表明蔗茅野生种的耐寒性均强于滇蔗品种(系)。
Using seven wild sugarcane (Erianthus fulvus Ness.) and its three offsprings with sugarcane hybrid materials (yunnan sugarcane varieties/lines) as the materials to determinate the physiological and biochemical indexes related to cold tolerance at the seedling stage in low temperature stress (3 ℃). The results showed: (1) Through the pole sorting method to calculate the electrical conductivity, the content of soluble sugar, MDA, proline, soluble protein, chlorophyll, and superoxide dismutase (SOD) activity, the weight coefficient are 0.109, 0.194, 0.148, 0.133, 0.108, 0.183,and 0.124,respectively.The weight coefficients of soluble sugar content and chlorophyll content were the highest, which indicated the two physiological indexes had stronger effects on cold-tolerance. (2) Under the stress of low temperature, the cold tolerance of 10 materials strength in the order: Zhemao99-3 〉 Zhemao99-2 〉 Zhemao U 91-2 〉 Zhemao99-4 〉 Zhemao99-1 〉 Zhemao90-29 〉 Zhemao 1 91-8 〉 Dianzhe04-14 〉 Dianzhe01-58 〉 Dianzhe02-39. In conclusion, the cold tolerance of wild sugarcane were better than domestic sugarcane cultivars.