采用模糊隶属函数和系统聚类的分析方法,研究抗旱处理期间,宿根甘蔗叶片的衰老情况以及质膜透性、脯氨酸、可溶性糖、丙二醛、水分含量、叶绿素、净光合速率(Pn)、胞间CO2浓度(Ci)、气孔导度(Gs)、蒸腾速率(Tr)、水分利用率(WUE)等生理生化指标对干旱胁迫的响应。结果表明:结合各种生理生化指标的模糊隶属函数值,利用系统聚类将8个甘蔗品种宿根时的抗旱性划分为3类;其中,新台糖10、粤糖93-159、新台糖22、桂糖11、闽糖69-421表现出较强的抗旱性,粤糖86-368表现出中度抗旱性,滇蔗01-58和崖城89-9表现出较弱的抗旱性,所得结果与形态指标基本一致。利用隶属函数和聚类分析进行宿根甘蔗抗旱性的综合评价,可避免单一指标的片面性和不稳定性,适用多品种之间的比较。
The method of membership function and cluster analysis were used to study the response of ratoon cane leaf senescence in the treatment, and the physiological and biochemical indicators such as the plasma membrane permeability, praline content, soluble sugar, malondialdehyde content (MDA), moisture content, chlorophyll content, net photosynthetic rate (Pn), intercellular CO2 concentration (Ci), stomata conductance ( Gs), transpiration rate ( Tr), water use efficiency (WUE) to drought resistance of ratoon cane. The results showed that combined with fuzzy membership function value of various physiological and biochemical indicators, by the cluster analysis, the eight varieties were divided into three categories, ROC 10, Yuetang 93-159, ROC 22, Guitang 11, Mintang 69-421 showed highly drought-resistance, Yuetang 86-368 showed moderately drought-resistance, Dianzhe 01-58 and Yacheng 89-9 showed weak drought-resistance, the results and morphological indices were basically the same. It was an effective way to comprehensively evaluate the ratoon cane by membership function and cluster analysis to avoid the bias and the instability of single factor analysis, which was suitable for many kinds of ratoon cane to compare.