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玉米幼胚胚性愈伤组织再生能力相关性状遗传研究
  • ISSN号:0253-9772
  • 期刊名称:《遗传》
  • 时间:0
  • 分类:S513[农业科学—作物学]
  • 作者机构:四川农业大学玉米研究所,成都611130
  • 相关基金:国家自然科学基金项目(编号:31471512)资助.
中文摘要:

本文对来源于美国、墨西哥和中国的144份不同玉米自交系幼胚胚性愈伤组织的再生能力相关性状进行了研究,发现其再生能力受到环境、基因型及环境与基因型互作三方面的影响。其中各性状之间的相关性表现为:绿点率(green embryonic callus rate, GCR)、分化率(embryonic callus differentiating rate, CDR)及再生绿苗数(the plantlet number of embryonic callus regeneration, CPN)之间呈极显著正相关,且这三者与褐化率(embryonic callus browning rate, CBR)呈极显著负相关;两次继代的克隆指数(embryonic callus cloning index forthe first subculture, CCI1; embryonic callus cloning index for the second subculture, CCI2)呈显著正相关,且CCI2与GCR有一定的正相关关系,与CBR呈负相关关系;生根率(embryonic callus rooting rate, CRR)则与GCR、CDR及CPN呈一定正相关。经过广义遗传力计算发现:胚性愈伤组织的两次继代克隆指数CCI1、CCI2和CRR的遗传力较低,其他性状的遗传力较高。此外,经Ward法双向聚类分析,共发现了11个具有高再生能力的自交系材料,且通过生根培养发现其再生绿苗的生根情况良好,因而可将它们作为玉米转基因受体的骨干自交系。

英文摘要:

We carried out a study of regeneration capacities of embryonic callus from maize immature cmbryo culture with 144 different inbred lines of natural groups from different countries, and found that the regenera- tion capacity was affected by three factors: environment, genotype and the interaction between the environment and genotype. We found that green embryonic callus rate (GCR), embryonic callus differentiating rate (CDR) and the plantlet number of embryonic callus regeneration (CPN) have significant positive correlations with each other, and they all have significant negative correlations with embryonic callus browning rate (CBR). Moreover, embryo- nic callus cloning index for the first subculture (CCI1) and embryonic callus cloning index for the second subculture(CCI2) have a significant positive correlation with each other, and CCI2 is positively correlated with green GCR, and is negatively correlated with CBR. Embryonic callus rooting rate (CRR) is positively correlated with GCR, CDR and CPN to some degree. Furthermore, we calculated Broad-Sense Heritability of each trait, and uncovered that the heri- tability index of CCI1, CCI2 and CRR was lower, and the heritability index of others was higher. In addition, by using the Ward method for two-way cluster analysis, we found eleven inbred lines with high regenerating abilities, and the rooting situation of regenerating plantlet was excellent by rooting culture, which could be used as the elite inbred lines of the maize transgenic receptor.

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期刊信息
  • 《遗传》
  • 中国科技核心期刊
  • 主管单位:中国科学院
  • 主办单位:中国遗传学会
  • 主编:张永清
  • 地址:北京朝阳区北辰西路1号院中国科学院遗传发育所
  • 邮编:100101
  • 邮箱:yczz@genetics.ac.cn
  • 电话:010-64807669
  • 国际标准刊号:ISSN:0253-9772
  • 国内统一刊号:ISSN:11-1913/R
  • 邮发代号:2-810
  • 获奖情况:
  • 中国自然科学核心期刊,《CAJ-CD》执行优秀奖,2008年12月获“中国精品科技期刊”证书和北京市印...
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,荷兰文摘与引文数据库,美国生物医学检索系统,美国生物科学数据库,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),中国北大核心期刊(2000版)
  • 被引量:23270