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利用磁镊研究促旋酶与DNA的结合
  • ISSN号：0023-074X
  • 期刊名称：《科学通报》
  • 时间：0
  • 分类：O312[理学—一般力学与力学基础;理学—力学]
  • 作者机构：[1]中国科学院物理研究所软物质物理重点实验室,北京凝聚态物理国家实验室,北京100190
  • 相关基金：国家自然科学基金（10834014,10974248）和国家重点基础研究发展计划（2009cB930704）资助
作者： 张志强[1], 刘玉如[1], 谢平[1], 李伟[1], 窦硕星[1], 王鹏业[1]
关键词： 促旋酶, DNA, 超螺旋, 单分子
中文摘要：

促旋酶（gyrase）在原核生物的生命活动中扮演着重要的角色,它是已知拓扑异构酶中,唯一能够向DNA引入负超螺旋的酶.本文中,我们利用磁镊（magnetic tweezers）对促旋酶和DNA的相互作用进行了单分子实时观测.实验发现,在不加入ATP时,促旋酶能够同时与两段DNA（G片段和T片段）结合,但这种结合较弱,施加0.7pN的外力就能逐渐破坏两者的结合;但加入高浓度的诺氟沙星后,促旋酶与DNA的结合明显增强,甚至能够抗衡5.9pN的外力.促旋酶还会影响超螺旋的几何尺度：不加入促旋酶时,DNA超螺旋的几何尺度由DNA所受拉力决定,而加入促旋酶后,超螺旋DNA几何尺度变小,并且主要由DNA与促旋酶的相互作用而不再是所受拉力所决定.而且促旋酶与正负超螺旋的结合能力不同,它更容易与正超螺旋结合.同时,发现促旋酶从DNA上解离的时间符合双指数分布.我们提出的模型能够很好地解释这个分布,并与他人提出的模型的结果进行了比较.

同期刊论文项目
非环形DNA解旋酶工作机理研究
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利用单分子和荧光方法研究生物大分子的动力学
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期刊信息
  • 《科学通报》
  • 北大核心期刊（2011版）
  • 主管单位:中国科学院
  • 主办单位:中国科学院
  • 主编：周光召
  • 地址：北京东黄城根北街16号
  • 邮编：100717
  • 邮箱：csb@scichina.org
  • 电话：010-64036120 64012686
  • 国际标准刊号：ISSN：0023-074X
  • 国内统一刊号：ISSN：11-1784/N
  • 邮发代号:80-213
  • 获奖情况:
  • 首届国家期刊奖,中国期刊方阵“双高”期刊,第三届中国出版政府奖
  • 国内外数据库收录:
  • 美国化学文摘（网络版）,美国数学评论（网络版）,美国工程索引,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊（2004版）,中国北大核心期刊（2008版）,中国北大核心期刊（2011版）,中国北大核心期刊（2014版）,中国北大核心期刊（2000版）
  • 被引量:81792