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中文摘要：
目的：探讨类叶升麻苷（Acteoside,AS）对APP/PS1双转基因小鼠老化、焦虑情绪、学习记忆及抗氧化能力的影响。方法：将9月龄的APP/PS1双转基因小鼠随机分为模型组、多奈哌齐组（3mg/kg）、类叶升麻苷低、中、高剂量组（20、40、80mg/kg）,每组10-11只,正常对照组为10只同月龄相同背景C57BL/6J小鼠。连续给药135天后,从老化评分、自主活动、O迷宫、穿梭箱实验检测小鼠老化程度、焦虑情绪及记忆能力;采用WST法检测小鼠血清中超氧化物歧化酶（SOD）抑制率,采用比色法测定丙二醛（MDA）、谷胱甘肽过氧化物酶（GSH-Px）水平;Luminex法测定了脑组织中BDNF含量。结果：老化评分结果显示,与模型组相比,类叶升麻苷（20、40、80mg/kg）组均能减缓APP/PS1小鼠衰老进程;与模型组相比,类叶升麻苷（80mg/kg）组小鼠躁狂情绪减弱;O迷宫结果显示,类叶升麻苷（20mg/kg）组能缓解小鼠焦虑情绪;类叶升麻苷可升高SOD抑制率和GSH-Px活性,使MDA含量降低;类叶升麻苷（80mg/kg）组有效改善小鼠垂体内BDNF的匮乏状态。结论：类叶升麻苷可通过延缓衰老、改善焦虑情绪,提高学习记忆能力,提高抗氧化能力等途径起到治防老年痴呆的作用。
英文摘要：
Objective：To investigate the effect of acteoside on aging, anxiety, learning and memory and antioxidant in APP/PS1 mice. Methods： 7-month old APP/PS1 mice were randomly divided into model group, donepezil group （3mg/kg） ,acteoside groups （20, 40, 80mg/kg）, 10 11 mice in each group. After intragastrical administration for 135 days, we detected the degree of aging, anxiety and memory ability of mice by Aging score, Autonomic activity experiment, O maze and Shuttle box test respectively; WST method was used to detect the inhibition rate of serum SOD and detecte contents of MDA and GSH-Px by colorimetry; BDNF content in brain tissue was determined by Luminex. Resuits： Aging scores showed that, acteoside（20, 40, 80mg/kg） groups slowed the aging process of APP/PS1 mice （P 〈 0.01, P 〈 0.01, P 〈 0.05 ） ; compared with model group, acteoside （80mg/kg） group can decrease mouse manic mood （ P 〈 0.05 ） ; O maze showed that Acteoside （20mg/kg） group mice can alleviate the anxiety （P 〈 0.01 ） ; The inhibition rate of SOD and GSH-Px activity were increased and the content of MDA was decreased （P 〈 0.01 ） ;acteoside（80mg/kg）effectively improved the lack of BDNF on mouse pituitary （P 〈 0.05 ）. Conclusion： Acteoside can anti-aging ,improve the anxiety, improve the ability of learning and memory , and can prevent and treatment Alzheimer disease by enhancing the antioxidation ability.
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