中文摘要：

为了研究SCBP60g的功能，研究利用Gateway克隆技术构建了GFP报告基因与拟南芥SCBP60g基因融合表达载体和基因功能互补表达载体并获得了转基因植物，通过激光共聚焦显微镜观察了SCBP60g蛋白的亚细胞定位情况，利用活体细菌生长量检测实验研究了SCBP60g蛋白在抗病中的功能；同时构建了SCBP60g过表达载体并检测了转基因植物对ABA的响应情况。结果显示，SCBP60g定位于细胞核；SCBP60g基因不能恢复CBP60g基因突变体对丁香假单胞菌的敏感性；过量表达SCBP60g基因不影响拟南芥对ABA的敏感性。表明SCBP60g没有参与拟南芥的抗病防御反应以及对ABA的响应。

英文摘要：

The GFP fused SCBP60g constructs and SCBP60g complementation constructs were made by Gateway cloning technology, and the transgenic plants were obtained. Subcellular localization SCBP60g was observed by confocal laser-scanning microscope, and the function of plant defense against pathogens was examined by in planta bacterial growth assay. In addition, SCBP60g overexpression vector was constructed and the sensitivity to ABA of the transgenic plants was also examined. The results showed ： SCBP60g protein localized in the nuclei; SCBP60g failed to restore the sensitivity of cbp6Og-1 mutant to Pseudomonas syringae; overexpression of SCBP60g did not affect the response of Arabidopsis to ABA. These results indicated that SCBP60g was not involved in Arabidopsis thaliana response to pathogens and ABA.