中文摘要：

ObjectiveTo 在骨头髓的增长上调查 icariin 的效果在 Sprague-Dawley (SD ) rats.MethodsBMSCs 的间充质的干细胞(BMSC ) 与微分时间从 SD 老鼠骨头髓被获得支持者方法。它的特征通过区别房间表面抗原和多系(osteo/adipo/chondo ) 被识别区别潜力。3-(4,5-Dimethylthiazol-2-yl )-2,5-diphenyltetrazolium 溴化物(MTT ) 方法和 5-Bromo-2-Deoxyuridine (BrdU ) 加入被使用在 BMSC 增长上检测 icariin 的效果。流动 cytometry 被用来检测 BMSC 的增长索引。mRNA 水平和 -catenin 的分发被实时聚合酶链反应(PCR ) 和分别地染色的 Immunofluorescent 评估。西方的污点被用来检测贝它(GSK-3 ) ， phospho 肝糖 synthase kinase-3 贝它(pGSK-3 ) 和 cyclinD1.ResultsIcariin 支持了的 -catenin, 肝糖 synthase kinase-3 的蛋白质表示层次在 0.05-2.0 mg/L 的集中的 BMSC 增长。BMSC 的 BrdU 积极房间的百分比从 40.98% ～ 70.42% 被增加，并且增长索引价值与 0.05 mg/L icariin 的处理从 8.9% ～ 17.5% 被增加，意义价值两个都是它不到 0.05。与控制组，全部、原子的 -catenin 蛋白质，以及 -catenin mRNA 相比表示，都与 icariin 处理被增加。同时， GSK-3 和 cyclinD1 蛋白质表情的 phosphorylation 水平也与现在的学习的调查结果表明了的 icariin treatment.ConclusionThe 在 BMSC 被增加 icariin 的那低剂量能支持 BMSC 增长。Wnt/-catenin 小径的激活涉及这个过程。

英文摘要：

OBJECTIVE： To investigate the effect of icariin on proliferation of bone marrow mesenchymal stem cells（BMSCs） in Sprague-Dawley（SD） rats.METHODS： BMSCs were obtained from SD rat bone marrow with differential time adherent method. Its characteristic was identified through differentiation cell surface antigens and the multi-lineage（osteo/adipo/chondo） differentiation potential. 3-（4,5-Dimethylthiazol-2-yl）-2,5-diphenyltetrazolium bromide（MTT） method and 5-Bromo-2-Deoxyuridine（Brd U） incorporation were applied to detect the effect of icariin on BMSCs proliferation.Flow cytometry was used to detect proliferation in-dex of BMSCs. The m RNA level and the distribution of β-catenin were evaluated by Real-time Polymerase Chain Reaction（PCR） and Immunofluorescent staining respectively. Western blot was used to detect protein expression levels of β-catenin, glycogen synthase kinase-3 beta（GSK-3β）, phospho-glycogen synthase kinase-3 beta（p GSK-3β）and cyclin D1.RESULTS： Icariin promoted BMSCs proliferation at the concentration of 0.05-2.0 mg/L. The percentage of Brd U positive cells of BMSCs was increased from40.98% to 70.42%, and the proliferation index value was increased from 8.9% to 17.5% with the treatment of 0.05 mg/L icariin, which significance values were both less than 0.05. Compared with the control group, total and nuclear β-catenin proteins, as well as β-catenin m RNA expression, were all increased with icariin treatment. Meanwhile, the phosphorylation level of GSK-3β and cyclin D1 protein expressions were also increased in BMSCs with icariin treatment.CONCLUSION： The findings of the present study demonstrated that low dosage of icariin could promote BMSCs proliferation. The activation of Wnt/β-catenin pathways was involved in this process.