中文摘要：

目的探讨ST段抬高型心肌梗死（STEMI）患者CD147基因3’非翻译区（UTR）rs8259位点多态性,并观察CD147的基因型及其血浆水平与STEMI的相关性。方法以162例STEMI患者和328例正常对照者作为研究对象,应用聚合酶链反应-限制性片长多态性（PCR-RFLP）技术和DNA测序法检测CD147基因rs8259位点的基因型,酶联免疫吸附实验（ELISA）检测血浆CD147水平。结果 STEMI组血浆CD147水平（4.18±0.95 pg/L）显著高于对照组（2.55±0.29 pg/L）（P〈0.01）。CD147基因rs8259位点存在3种基因型（AA、AT、TT）,基因型和等位基因频率在STEMI组和对照组分布差异有显著性（P〈0.05）。对多个危险因素行Logistic回归分析显示AT基因型（OR=0.346,95%CI：0.210～0.569,P〈0.05）和TT基因型（OR=0.107,95%CI：0.046～0.251,P〈0.05）能降低STEMI发病易感性的风险。T等位基因携带者患STEMI的相对风险度明显降低（OR=0.543,95%CI：0.404～0.730,P〈0.05）。A等位基因携带者患STEMI的相对风险度升高（OR=1.841,95%CI：1.370～2.464,P〈0.05）,携带T等位基因的STEMI患者血浆CD147水平相比于不携带者明显降低（P〈0.05）。结论 CD147基因3’UTR rs8259 A等位基因可能是STEMI发病的易感基因;AA基因型促进CD147高表达进而增加STEMI发病风险,携带T等位基因通过降低CD147表达而降低STEMI的发病风险。

英文摘要：

Aim To analyze the polymorphism of the CD147 gene 3’UTR rs8259 in patients with ST-segment elevation myocardial infarction（STEMI）,and to study the relation between plasma levels,genotype of CD147 and STEMI.Methods The polymorphism of CD147 was detected by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） and DNA sequencing method in 162 STEMI patients and 328 healthy persons. The plasma level of CD147 was determined by enzyme-linked immunosorbent assay（ ELISA）. Results STEMI group（4. 18 ± 0. 95pg /L） showed significantly higher plasma level of CD147 than control group（2. 55 ± 0. 29 pg /L）（ P 〈 0. 01）. There was a significant difference in frequencies of alleles and genotypes in 3’UTR rs8259 of CD147 with three genotypes AA,AT and TT existed（P 〈 0. 05）. Logistic regression analysis for adjusting other risk factors displayed that AT genotypes（OR= 0. 346,95% CI： 0. 210 ～ 0. 569,P 〈 0. 05） and TT genotypes（OR = 0. 107,95% CI： 0. 046 ～ 0. 251,P 〈 0. 05） can decrease the relative risk of STEMI. Allele T carriers had low onset risk for STEMI（ OR = 0. 543,95% CI： 0. 404 ～0. 730,P 〈 0. 05）,and allele A carriers had high risk for STEMI（OR = 1. 841,95% CI： 1. 370 ～ 2. 464,P 〈 0. 05）.The plasma level of CD147 was the highest in AA genotype（P 〈 0. 05）. Conclusions CD147 gene 3’UTR rs8259 allele A is probably the susceptible gene of STEMI. AA genotype can be at increased risk of STEMI due to enhancing the CD147 expression and allele T may be protective for STEMI.