中文摘要：

研究表没食子儿茶素没食子酸酯（EGCG）的甲基化分子修饰。以碘甲烷作为甲基供体,采用化学合成方法研究EGCG的甲基化分子修饰,并通过HPLC-MS和NMR等对反应产物进行结构鉴定。结果表明：采用化学合成方法能有效完成EGCG的甲基化分子修饰,并分离鉴定出5个EGCG甲基化衍生物,分别为4″-Me-EGCG、4′,4″-di-Me-EGCG、5,3′,4′,5′,3″,4″,5″-hepta-Me-EGCG、5,7,3′,4′,3″,4″,5″-hepta-Me-EGCG、5,7,3′,4′,5′,3″,4″,5″-octa-Me-EGCG。

英文摘要：

For EGCG methylation via chemosynthesis,methyl iodide was used as methyl donor and two reaction systems（No.1 and No.2） were designed,in which the molar ratios between EGCG and methyl iodide were set at 1：80 and 1：1,respectively.Reaction products TRI-1 and TRI-2 obtained from reaction system No.1 and No.2,respectively were characterized by HPLCMS and NMR.The results showed that the molecular modification of EGCG could be successfully accomplished by the use of chemosynthesis,and five EGCG derivatives,namely 4″-Me-EGCG,4＇,4″-di-Me-EGCG,5,3＇,4＇,5＇,3″,4″,5″-hepta-MeEGCG,5,7,3＇,4＇,3″,4″,5″-hepta-Me-EGCG and 5,7,3＇,4＇,5＇,3″,4″,5″-octa-Me-EGCG were identified in the two reaction products,of which,3 were contained in reaction product TRI-1 and 2 in reaction product TRI-2.