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中文摘要：
目的：研究激活素受体样激酶1（ALK1）对人脐静脉内皮细胞的作用。方法：体外培养人脐静脉内皮细胞（HUVECs），RT-PCR分析ALK1和ALK5在HUVEC激活状态下表达的变化。脂质体转染pcDNA3．1＋ALK1到HUVECs，流式细胞仪检测HUVECs增殖的改变，boyden小室检测ALK1对HUVECs迁徙的影响。结果：ALK1在HUVEC安静状态高表达，ALK1能促进HUVECs的增殖和迁徙。结论：ALK1通过促进内皮细胞的增殖和迁徙在血管重塑中发挥作用。
英文摘要：
AIM： To study the function of activin receptor- like kinase Ⅰ （ALK1） gene in vascular endothelium. METHODS： The human umbilical vein endothelial cells （HU3/ECs） were cultured, and the change of expression of ALK1, in activation of HUVECs was analyzed. The full - leng coding sequence of ALK1 was cloned into peDN/L3.1 ＋ using standard protocols. The construeted pcDNA3.1 ＋ ALK1 plasmid were transfeeted into HUVECs. The proliferation and migration of HUVECs were detected by boyden champer and flow cytometry. RESULTS： The expression of ALK1 was up- regulated in resolution. ALK1 prorooted the proliferation and migration of HUVECs. CONCLUSION： ALK1 has an important function in remodeling by promoting the proliferation and migration of endothelial cells.
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