中文摘要：

研究了不同金属离子及其添加形式对肺炎克雷伯氏菌胞内酶和胞外酶降解三苯基锡的影响,以期为阐明有机锡的酶促降解机制提供实验依据.结果表明,菌体对K＋、Mg2＋、Cu2＋、Ca2＋、Fe3＋具有良好的耐受能力,高浓度的Zn2＋、Fe2＋可能对菌体产生一定的毒害作用,从而影响其生长.适当浓度范围的K＋、Mg2＋、Zn2＋、Cu2＋、Fe2＋加入均能促进三苯基锡的酶促降解,其中30 mg/L Mg2＋的促进作用最明显,降解率为77.22%.一定浓度范围内的Fe3＋对酶促降解具有适当的抑制作用.K＋、Mg2＋、Zn2＋、Cu2＋加入培养基中,均可以促进其产酶效果,其中Mg2＋加入后降解效果最好,降解率达到85.66%,说明部分金属离子在适当的浓度范围内可以作为酶的激活剂促进三苯基锡的酶促降解.此外,金属离子对菌体生长量的影响和产酶效果没有相关的联系.某些金属离子只能作为胞内酶的激活剂而对胞外酶不能表现出相同作用.

英文摘要：

The influence of different metal ions and different forms of addition on triphenyltin enzymatic degradation was investigated under conditions using enzyme obtained from Klebsiella pneumoniae.The objective of this study is to illuminate the mechanism of enzymatic degradation of triphenyltin（TPhT）.The results demonstrated that the strain was able to tolerate K ＋,Mg2 ＋,Cu2 ＋,Ca2 ＋ and Fe3 ＋ at high concentrations.High concentrations of Zn2 ＋ and Fe2 ＋ had some toxic effects on the strain,thus affecting its growth.The endoenzyme activity was enhanced by metal ions such as K ＋,Mg2 ＋,Zn2 ＋,Cu2 ＋ and Fe2 ＋ at certain concentrations.In the presence of 30 mg /L of Mg2 ＋,the removal percentage of TPhT was up to 77.22%.Fe3 ＋ restrained the enzyme activity at certain concentrations.Adding K ＋,Mg2 ＋,Zn2 ＋,Cu2 ＋ into medium can promote the production of enzyme,among which Mg2 ＋ demonstrated up to 85.66% of removal percentage of TPhT,suggesting some metal ions at the appropriate concentration range can be used as enzyme activator for the enzymatic degradation of triphenyltin.Metal ions showed no relevant impact on the cell growth and enzyme production.Certain metal ions can only serve as activators of endoenzyme and exhibited no similar effect towards exoenzyme.