中文摘要：

非变性荧光原位杂交（ND-FISH）是近年来兴起的一种技术，一般使用带有荧光标记的简单序列重复（SSRs）作为探针，杂交中不需要进行探针和染色体的变性使得检测简单快捷。SSRs探针可检测出染色体上SSR富集的区域，可以帮助辨识染色体，SSRs分布的多态性还可以为遗传多态性的研究提供信息。（1）该研究首次将ND-FISH运用于两栖动物中，得到了5个可运用于多态性研究的标记。（2）研究发现，有别于其他类群，当ND-FISH应用于两栖动物时，染色体标本必须进行变性。（3）该研究还对双色荧光原位杂交流程进行了较大的改进，在一个杂交体系中同时使用直接和间接标记探针，通过一次实验获得双色信号，从而使步骤简化。

英文摘要：

The recently popularized non-denaturing fluorescence in situ hybridization （ND-FISH） is a new technique that is both quick and efficient, in part because denaturing of both of the probes and the chromosomes is unnecessary. Synthetic simple sequence repeats （SSRs） labeled with fluorescein are used as probes to detect SSR-enriched chromosome regions and provide markers to identify the chromosomes. To date this method has not been applied to amphibians, even though the polymorphism of the distribution of SSRs may help to advance genetic polymorphism research. This paper also improved the double-colour FISH method by simultaneously using probes labelled with fluorescein and probes labelled with DIG to get double-color signals. This study found 5 SSRs markers that may be useful in the polymorphism research, and that the amphibian chromosomes must be denatured in ND-FISH.