中文摘要：

目的探讨功能化自组装纳米多肽DGEAmx水凝胶对小鼠骨髓间充质干细胞（mesenchymal stem cells,MSCs）的粘附、增殖和成骨分化的影响。方法原子力显微镜观察功能化自组装多肽形成的纳米级结构特征;用倒置显微镜对贴壁细胞拍照计数,对比细胞粘附情况;用CCK-8法检测细胞增殖;激光共聚焦显微镜观察细胞在材料表面形貌;检测碱性磷酸酶（alkaline phosphatase,ALP）活性、成骨分化特异性基因Ⅰ型胶原（ICAM-1）和骨钙蛋白（osteocalcin,OCN）的表达来评价成骨效果。结果原子力显微镜观察功能化自组装多肽DGEAmx可以形成纳米纤维网状结构;倒置显微镜对贴壁细胞拍照计数结果显示在30、60、90 min时DGEAmx组对比RADA16-Ⅰ组粘附细胞数差异有统计学意义（P〈0.05）;CCK-8法检测结果显示在培养第3、5、7天,DGEAmx组MSCs增殖较RADA16-Ⅰ组明显增高（P〈0.05）;DGEAmx组成骨诱导3、7、14 d后,ALP活性高于RADA16-Ⅰ组（P〈0.05）;Real-time PCR结果显示成骨诱导3、7、14 d后DGEAmx组较RADA16-Ⅰ组有更高的ICAM-1和OCN的表达（P〈0.05）。结论功能化自组装多肽DGEAmx纳米纤维水凝胶支架能促进MSCs粘附、增殖和成骨分化,作为骨组织工程支架材料有良好的应用潜力。

英文摘要：

which is prepared Ala） peptide, on （MSCs） in mice. the functionalized ceils and compare Objective To investigate the effect of functionalized self-assembling peptide DGEAmx, by modifying C terminal of self-assembling peptide RADA16-I with I）GEA （Asp-Gly-Glu- the cell adhesion, proliferation and osteogenic differentiation of bone mesenchymal stem cells Methods An atomic force microscope was used to detect nanostructure characteristics of self-assembling peptide DGEAmx. An inverted microscope was employed to count adherent cell adhesion. The proliferation of the MSCs was observed bv cell counting kit （CCK） -8 assay. Cell morphology was observed with a laser confocal microscope. Alkaline phosphatase （ALP） activity and expression of osteogenic differentiation-specific genes collagen I （ICAM-1） and osteocalcin （OCN） were detected to evaluate osteogenic differentiation of the MSCs. Results The atomic force microscope observation found that functionalized self-assembling peptide DGEAmx was capable of forming nanofiber network structure. Cell counting result showed that the adherent cells on the DGEAmx scaffold were more than those on the RADA16-I scaffold at 30, 60 and 90 min （P 〈 0.05 ）. CCK-8 assay results showed that the proliferation of the MSCs on the DGEAmx scaffold was significantly increased on days 3, 5 and 7 （P 〈 0. 05 ）. The MSCs cultured on the DGEAmx scaffold showed higher ALP activity and higher ICAM-1 and OCN expressions compared with those on the RADA16-I scaffold on days 3, 7 and 14 （P 〈 0. 05）. Conclusion The functionalized self-assembling peptide DGEAmx nanofiber hydrogel scaffold can promote the adhesion, proliferation and osteogenic differentiation of the MSCs, and has good potential as bone tissue engineering scaffold.