目的探索湖沼地区钉螺调查时确定适宜样本量的方法,为获取准确的钉螺信息提供理论依据。方法从安徽省贵池区秋浦河沿岸随机选择4块滩地,按照等间隔的抽样方法调查钉螺,所有钉螺带回实验室用水测法判别死活,并记录活螺数。用EpiData双遍录入校对建立数据库,用SAS软件对4块滩地的钉螺数据按不同的样本量进行抽样并计算相应的钉螺密度,绘制样本量-钉螺密度绝对变化曲线和样本量-钉螺密度相对变化曲线,判断各滩地的适宜样本量。结果谷潭湖滩的适宜样本量为40框,菜籽湖滩为200框,秋浦河外滩为100框,欧阳湖滩为200框。结论样本量-钉螺密度绝对变化曲线和相对变化曲线相结合可以为确定湖沼地区钉螺调查时的适宜样本量提供一定的指导作用。
Objective To explore a possible method for determining the appropriate sample size of Oncomelania hupensis in the marshland and lake regions. Methods Four bottomlands were selected randomly along the Qiupu River in Guichi District, Anhui Province. Oncomelania snails were surveyed carefully in these bottomlands and brought back to the laboratory to judge whether they were alive or not with mild water. All the data of Oncomelania hupensis were doubly input into the EpiData software and checked for the errors. Repeated sampling was performed in the four databases according to different sample sizes by using SAS software and the corresponding snail density was also calculated. According to the values of sample size and corresponding snail density, sample sizesnail density absolutely changing curve and relatively changing curve were drawn to determine the appropriate sample size of snail survey. Results The appropriate sample sizes of snail survey were 40,200,100 and 200 frames in Gutan bottomland, Caizi bottomland, Qiupu bottomland and Ouyang bottomland, respectively. Conclusion Sample size-snail density absolutely changing curve and relatively changing curve could provide some instructions for choosing the appropriate sample size during the process of snail survey in the marshland and lake regions.