中文摘要：

植物在感知到虫害信号后会经过复杂的转录调控,开启防御基因的表达。这一过程大多受到茉莉酸信号途径的调控,现有的相关研究主要集中在双子叶模式植物拟南芥中,在单子叶植物玉米中是否具有相似的转录调控模式目前尚不清楚。为了探索这一问题,从被玉米螟咬食和150μmol/L JA处理12 h的RNAseq玉米文库中获得两个同时受诱导的基因,Zm281和Zm Pal,重点对其启动子的诱导活性进行了分析。结果表明,这两个玉米启动子在未受JA处理的拟南芥中不能启动GUS报告基因的表达,但是在茉莉酸诱导后GUS报告基因受到强烈诱导,且在各组织器官中均有表达。该结果表明受虫咬后诱导表达的基因同时受JA的诱导,可为研究JA诱导虫害应答的分子机理奠定基础。

英文摘要：

Upon being attacked by herbivores, the plant defense response is triggered, which often involves complex transcriptional regulation of the defense genes. This process depends on a plethora of defense-related transcription factors and herbivory-inducible cis-elements on the promoters of defense genes, and is largely modulated by the jasmonate pathway. Current studies on these cis-elements focus on the model dicot Arabidopsis, and it is unknown whether they are also conserved in monocot crops. In order to explore the answer, this paper characterized the activity of 2 jasmonate- and herbivory-inducible maize promoters in Arabidopsis. It was found that these 2 promoters could not drive GUS expression in untreated Arabidopsis, but strong GUS expression could be detected in various tissues, when plants were treated with jasmonate. These results suggested that the expressive gene induced after being bitten by insect was at the same time induced by JA. This result would lay a foundation for studying the response molecular mechanism of JA induced herbivore.