中文摘要：

以壳聚糖为功能基体,尿酸为模板分子,利用恒电位沉积法制备对血清中尿酸具有高度选择性的分子印迹电化学传感器.以衰减全反射红外光谱（FTIR-ATR）和电化学交流阻抗法（EIS）等方法表征印迹膜的形成,并应用伏安技术研究该传感器的电化学行为.研究结果表明：在0.1mol.L-1（pH=5.0）的磷酸盐（PBS）缓冲溶液中,尿酸在该印迹传感器上具有良好的电化学响应,氧化峰电流与尿酸的浓度在0.1～80.0μmol.L-1之间呈良好的线性关系,相关系数为0.999 1.所制备的传感器具有良好的选择性,稳定性和重现性,将该传感器应用于实际样品中尿酸的分析检测,方法回收率在97.41%～102.8%之间.

英文摘要：

A highly selective molecularly imprinted electrochemical sensor of uric acid（UA） was prepared with chitosan（CS） as functional matrix and uric acid as template molecule via constant potential electrochemical deposition.The imprinted membrane was characterized by Attenuated total reflection infrared spectroscopy（ATR-FTIR） and Electrochemical impedance spectroscopy（EIS）;the performances of the sensor were studied by cyclic voltammetry（CV） and differential pulse voltammetry（DPV）.The UA had good electrochemical response in 0.1 mol·L-1 phosphate buffer solution（PBS）（pH=5.0）.The oxidation peak current of DPV was well-proportional to the concentration of UA in the range from 0.1 μmol·L-1 to 80.0 μmol·L-1,with a correlation coefficient of 0.999 1.The developed sensor exhibited specific recognition for UA against the competitors which consisted of similar structure and coexisting interference in human serum.Moreover,the sensor also shows exellent reproducibility and was successfully employed to the determination of UA in human serum with the recovery of 97.41%~102.8%.