中文摘要：

目的探讨R，S和R／S型3-氯-1，2-丙二醇（3-MCPD）对ICR小鼠的急性毒性。方法选用健康性成熟ICR小鼠，对R，S及R／S型3-MCPD染毒剂量依次为176．78、198．43、222．73、250、280．61、314．98和353．55、396．84、445．44和499．99mg／kg，89．09，100，112．25，125．99，141．42，158．74和178．18mg/kg及130．25、150、172．75、198．95、229．13、263.88和303．91mg／kg。经口一次灌胃染毒观察14天，以改良寇式法计算LD50，计算脏体比并进行肝肾病理学检查。结果R，S及R／S型3-MCPD的LD50及95％可信区间（95％CI）分别为290．54mg／kg（280．74～300．68），117．57mg／kg（113．82，121．45），190．73mg／kg（177．76～204．59）。R型异构体在250mg／kg及以上剂量组动物肾体比显著增加（P〈0．05），脑体比在353．55mg／kg、445．44mg／kg及最高剂量组亦显著增大（P〈0．05）；S型3-MCPD染毒组动物各脏体比与对照组比均无显著差异；R／S型3-MCPD，在198．95mg／kg及以上荆量组动物肾体比明显增加，在229．13mg／kg及以上剂量动物脑体比亦明显增加，与对照组比有差异显著（P〈0．05）。在353．55mg/kg及以上剂量R型异构体引起肝细胞肿胀、肝窦扩张和明显充血，在229．13mg/kg及以上荆量（R／S）型3-MCPD也引起肝细胞肿胀和肝窦充血，S型则未引起。R、S及（R，S）型均未引起肾脏明显的病理改变。结论三种3-MCPD急性毒性大小依次为S型〉R／S型〉R型，R、S型异构体均显示出神经毒性。

英文摘要：

Objective To investigate the acute toxicity of R,S and （R,S） -3-monchloropropane-1,2-diol（3-MCPD）. Methods 10,7 and 7 groups of sexually mature ICR mice were employed for testing the acute toxicity of R, S and （R, S）-3-MCPD, at the doses of 176.78,198.43,222.73,250,280.61,314.98 and 353.55mg/kg 396.84mg/kg, 445.44mg/ kg,499.99mg/kg for R-3-MCPD, 89.09,100,112.25,125.99,141.42,158.74 and 178.18mg/kg for S-3-MCPD and 130.25,150,172.75,198.95,229.13,263.88 and 303.91 mg/kg for （ R, S）-3- MCPD, respectively. The mice were given three forms of 3-MCPD once by gavage and observed for 14 days, the lethal dose 50s（LD50） were calculated by the modified Karber＇s method, organ/body weight ratios were measured and morphological changes of liver and kidney were examined. Results The LD50 （95% Cl）of R, S and （R,S）-3-MCPD were 290.54mg/kg（280.74 - 300.68）, 117.57mg/ kg （ 113.82 - 121.45 ） and 190.73mg/kg（ 177.76,204.59）, respectively. The kidney/weight ratios in R-3- MCPD treated groups were higher than controls at the doses of 250 - 353.55mg/kg （P 〈 0.05）, and the brain weight ratios also had an significant increase in the 353.55mg/kg,445.44mg/kg and above doses （P 〈 0.05 ）. However, no significant change on organ/body weight ratio of any organs in S-3-MCPD treated groups were observed. The kidney/body weight ratio in （R, S）- 3-MCPD treated groups increased significantly at the doses of 198.95 - 303.91mg/kg（ P 〈 0.05）, and the brain weight ratios also had a significant increase at the doses of 229.13～ 303.91mg/kg. At the doses of 353.55 ～ 499.99mg/kg, R-3- MCPD caused obvious swell of liver cells and the swell and congestion of the liver sinus. At the dose 229.13mg/kg and above, （R, S）-3-MCPD also induced the swell of liver cells and congestion of the liver sinus. No obvious morphological changes of kidney after administration of R, S and （R, S）-3-MCPD were observed. Conclusion The results indicated that the acute toxicity of S-3-MCPD may be much higher