中文摘要：

目的观察芍药苷（抗炎类中药活性成分）对肿瘤坏死因子-α（TNF-α）诱导的人成纤滑膜细胞（FLS）功能的影响。方法体外培养人FLS，考察TNF-α浓度在0．02—20μg·L^-1时对FLS的增殖反应和FLS分泌前列腺素E2、环磷腺苷和白介素-1β的影响；加入芍药苷（1×10^-8～1×10^-4mol·L^-1）后，观察对其增殖反应和分泌功能的影响；用MTT法检测FLS的增殖反应，用放射免疫测定法检测白介素-1β，用酶联免疫吸附法检测前列腺素E2和环磷腺苷的水平。结果TNF-α浓度在0．02～20．00μg·L^-1时，均显著抑制FLS分泌环磷腺苷，显著促进前列腺素E2和白介素-1β的分泌；芍药苷能显著抑制TNF—α对FLS的促增殖反应，明显上调降低的环磷腺苷水平，明显下凋升高的前列腺素E2和白介素-1β水平，且具有一定的浓度依赖性。结论TNF-α可以促进FLS的增殖，降低环磷腺苷，升高前列腺素E2和白介素-1β；芍药苷则能明显逆转TNF-α对FLS的影响。

英文摘要：

Objective To investigate effects of paeoniflorin （Pae） on functions of fibroblast - like synoviocytes （FI,S） of human under the stimulation of tumor necrosis factor - α（TNF - α）. Methods FLS of human were cultured in vitro. The TNF - α of 0. 02 - 20. 00 μg · L^-1 was incubated with the FLS to measure the proliferative rate of FLS by a MTF assay and levels of interleukin - 1β （ IL - 1β ） by radioimmunoassay;prostaglandins E2 （PGE2） and cyclic adenosine monophosphate （cAMP）by ELISA. The added - on effect of Pae（1 × 10^-8 ～ 1 × 10^-4 mol · L^- 1 ） on the proliferative human of FLS and levels of PGE2 , cAMP and IL - 1β were determined at the concentration of TNF - α （20 μg · L^-1 ） . Results TNF - α （ 0. 02 ～ 20.00 μg · L ^- 1 ） significantly all stimulated the proliferation of the cultured FLS, reducing level of the cAMP and increasing the levels of PGE2 and IL - 1β. Pae suppressed the TNF- α stimulation on FLS, manifesting the up-regulation of the reduced cAMP level and down -regulation of the increased PGE2 and IL- 1β levels in a concentration - dependent manner. Conclusion Pae showed a capacity to reverse the TNF - α stimulation on the FLS of human.