中文摘要：

目的探讨不同真菌菌种与人角膜上皮细胞黏附过程中整合素的表达及其作用机制。方法实验研究。体外培养人角膜上皮细胞系，建立茄病镰刀菌（CGMCC 3．1829）和烟曲霉菌（CGMCC 3．0772）与角膜上皮细胞黏附的体外模型。茄病镰刀菌或烟曲霉菌与人角膜上皮细胞共孵育后，用无菌的磷酸盐缓冲溶液冲洗掉未黏附的真菌。提取细胞的总RNA，反转录为cDNA后采用实时荧光定量聚合酶链反应（RT-PCR）检测不同时间点上不同真菌与角膜上皮细胞黏附的14种整合素分子mRNA水平的表达。各时间点之间基因表达差异的比较采用单因素方差分析。结果在烟曲霉菌与人角膜上皮细胞黏附的过程中，随黏附时间延长，整合素家族白细胞黏附受体组成员编码基因整合素αL（ITGAL）、整合素α型（ITGAM）、整合素αX（ITGAX）及整合素β2（ITGB2）的表达显著上调。其中ITGAL的表达最高上调2倍（F=29．39，P〈0．01），ITGAM的表达最高上调4倍（F=20．26，P〈0．01），ITGAX的表达最高上调2．5倍（F=2．51，P〈0．05），ITGB2的表达最高上调3．4倍（F=3．923，P〈0．05）。而在茄病镰刀菌与人角膜上皮细胞黏附的过程中，此14种整合素的表达未见显著差异。结论整合素家族白细胞黏附受体组（B2组）成员αLβ2、αMβ2及αXβ2均参与烟曲霉菌与角膜上皮细胞的黏附；未见茄病镰刀菌与角膜上皮细胞的黏附过程中整合素表达的差异。整合素介导的黏附因菌种而异。

英文摘要：

Objective To investigate the expression of integrin during the course of adhesion between fungi and corneal epithelial cells. Methods It was an exprimental study. Human corneal epithelial cells were cultured and the adhesion model were established between human corneal epithelial cells and Fusarium solani （ CGMCC 3. 1829） or Aspergillus fumigatus （ CGMCC 3. 0772） in vitro. Human corneal epithelial cells were incubated with Fusarium solani or Aspergillus fumigatus at 37 ℃ and the non-adhered fungi were washed out with steriled phosphate buffered solution （PBS）. The total RNA was extract from the human corneal epithelial cells. Real-time polymerase chain reaction （RT-PCR） for the expression of integrin was performed. Results The results of RT-PCR showed that during the course of adhesion between Aspergillusfumigatus and corneal epithelial cells, the expression of integrin alpha L（ ITGAL）, integrin alpha M （ ITGAM ）, integrin alpha X （ITGAX） and integrin beta 2 （ ITGB2 ） were upregulated signigicantly. However, during the course of adhesion between Fusarium solani and corneal epithelial cells, there was no significant differences in the expression of those 14 kinds of integrin. Conclusions Integrin αLβ2, αMβ2 and αXβ2 are all belong to the leukocyte adhesion receptor group （ β2 group） in integrin family. These three members are all involved in the adhesion of Aspergillus fumigatus and corneal epithelial cells. With the adhesion, the expression of integrin is upregulated. These results suggest that the integrin-mediated adhesion is different with different kinds of fungi. Integrin plays an important role in adhesion of fungi with human corneal epithelial cells.