中文摘要：

单向不亲和对于研究种间花粉不亲和及自交不亲和机制有重要意义。以潘那利番茄‘LA0716’渐渗系群体中的单向不亲和株系为主要材料,通过花粉萌发试验,正、反交试验和花粉管在雌蕊中的伸长研究,发现单向不亲和株系的花粉活力正常,其花粉与普通栽培番茄杂交可正常结实,但是其雌蕊不能接受自身或栽培番茄的花粉。然而,将秘鲁番茄‘LA3858’的花粉授予单向不亲和株系,花粉能够正常萌发,花粉管正常伸长和受精,但是杂交种子败育。利用20个分子标记对BC5S3群体的1 000株植株进行遗传分析,结合表型鉴定结果,将单向不亲和基因UI3a精细定位于第3号染色体上分子标记C2_At3g10220和1k-1294之间,物理距离为349 kb,该区域编码37个基因。

英文摘要：

Unilateral incompatibility（UI）is a kind of incompatibility that occurs in one direction only,most often when the pollen donor is self-compatible（SC）and the recipient is self-incompatible（SI）.In the present study,we identified 4 unilateral incompatibility lines（ui3a）from an introgression line（IL）population of Solanum pennellii＇LA0716＇in a fresh market tomato inbred line 1052 that was developed by our group.Pollen germination test showed that the pollen viability of these ui3 a lines was normal.Crosses were successful when these ui3a lines as pollen donor and the self-compatibility（SC）lines from the IL population as recipient,but not vice versa.Pollen tube growth studies indicated that the pistils of these ui3a lines rejected the pollen of themselves and self-compatibility（SC）lines from the IL population,but accept the pollen of S.peruvianum accession ＇LA3858＇.Subsequently,UI3 a gene was fine-mapped to a 349 kb region between markers C2_At3g10220 and 1k-1294 on chromosome 3.There were 37 predicted genes in this region.