中文摘要：

目的：探讨高住低训（HjLo）对大鼠骨骼肌中低氧诱导因子-1α（HIF-1α）和血管内皮生长因子（VEGF）基因表达的影响。方法：96只7周龄sD大鼠随机分为4组：安静对照组、低氧对照组、常氧训练组和高住低训组。每组又分为3小组，分别是4、7和28天组。高住低训模型：在模拟海拔4km（12．7％O2）的常压低氧舱居住，以中等强度（相当于55％-60％VO2max）进行常氧训练。采用RT—PCR法检测，半定量分析大鼠腓肠肌HIF-1αmRNA和VEGF mRNA表达。结果：在4周观察期内，（1）单纯低氧或常氧训练引起的VEGF mRNA变化趋势与HIF-1α mRNA大致吻合：开始迅速升高，然后随时间推移逐渐下降，但第28天时仍略高于对照组。提示：低氧或常氧训练诱导的VEGF转录表达，可能通过以HIF-1α为核心转录因子的低氧信号转导通路来调节。（2）高住低训能诱导腓肠肌HIF-1α和VEGF mRNA表达，但其升高趋势会因时间差异而不同。提示，HiLo对HIF-1α和VEGF基因表达的影响，不是低氧和训练两种刺激的简单叠加，而是综合复杂作用的结果。

英文摘要：

Objective The purpose of the study was to investigate the effect of living at high altitude and training at low altitude（HiLo） on the HIF-1α and VEGF mRNA expressions in skeletal muscles. Methods Eighty 7 - wk - old male SD rats were randomly assigned to 4 groups： control（C） ,hypoxic control（HC）, normoxic training（NT） and hypoxic living ＋ normoxic training（HT）. Each group was divided into 3 subgroups, the 4 - day, 7 - day and 28 - day. A HiLo model was designed to simulate living at 4km altitude while training under normoxia with moderate intensity. RT - PCR was employed to measure the HIF-1α and VEGF mRNA expressions in gastrocnemii. Results and Conclusions During the experiment, （ 1 ） the trend of VEGF mRNA was roughly consistent with that of HIF-1α during hypoxia or normoxic training： VEGF mRNA increased rapidly at the beginning, and then slowed down as time elapses, but still slightly higher than the baseline on day 28. These suggest that either hypoxia or normoxic training induced the transcription of VEGF through HIF -1α, the key regulator of O2 - sensing pathway; （2） HiLo could induce mRNA expressions of HIF -1α and VEGF in the gastrocnemii, but the increasing trend with time course was different. The effects of HiLo on gene expressions of angiogenesis factors were not a simple additive outcome of the two stimulations, but a combination of intricate factors.