中文摘要：

单细胞油脂是生产生物柴油最理想的原料,随着化石能源的日益枯竭,单细胞油脂的生产受到广泛关注。解脂耶罗维亚酵母是生产单细胞油脂的最佳菌株,它能够利用诸多廉价底物作为碳源,在工业上有极大的应用前景。其遗传背景清晰,全基因组测序已完成,基因表达系统已构建。在此基础上对油脂累积途径进行了深入研究,多株油脂含量更高的菌株被构建。深入了解解脂耶罗维亚酵母的基因表达及油脂代谢系统,对日后对其进一步的代谢改造具有重要意义。

英文摘要：

Single cell oil（ SCO） is the most ideal material for biodiesel production. With the increasing depletion of fossil fuels,the production of SCO has been widely concerned. Yarrowia lipolytica is one of the best strain for the production of SCO. Many kinds of cheap substrates can be used as carbon source,so it has great application in industry. Its genetic background is clear,the whole genome sequencing has been completed,gene expression system has been constructed. On the basis of this,the oil accumulation ways were studied,and the strains with higher oil content were constructed. It has great significance to research the gene expression and lipid metabolism of Y. lipolytica for further study.