中文摘要：

目的探讨小分子RNA干扰（siRNA）沉默p65基因的表达对胶质瘤细胞生长和糖代谢的影响。方法应用RNA干扰技术降低p65表达后,用四甲基偶氮唑盐微量酶反应比色法和流式细胞术检测胶质瘤细胞的增殖和周期,Western blot检测p65下游功能蛋白的变化。结果抑制p65的表达后,U87和U251细胞生长受抑制,细胞生长阻滞在G1期且细胞糖代谢水平下降,下游的功能蛋白周期素D1和丙酮酸激酶M2（PKM2）表达水平降低。结论干扰p65的表达可以抑制胶质瘤细胞的生长和糖代谢水平。

英文摘要：

Objective To explore the effects of p65 silence by small molecular RNA interference （p65-siRNA） on glioma cell growth and glucose metabolism. Methods After p65 expression was reduced by siRNA, the growth and cycle of glioma cells U87 and U251 were examined with MTT assay and flow cytometry, and the change of downstream function protein of p65 was detected by Western blot. Results After reduction of.p65 expression, the growth of U87 and U251 cells was suppressed and blocked in G1 phase with reduced glucose metabolism and the expressions of cyclin D1 and PKM2 in downstream function protein of p65. Conclusion The p65-siRNA can effectively suppress the glioma cells growth and glucose metabolism.