中文摘要：

通过对刚毛柽柳转录组分析,鉴定获得1个液泡膜H^＋-PPase基因的cDNA序列,命名为ThVP1。该cDNA序列全长3 022bp,开放阅读框为2 298bp,编码765个氨基酸,编码蛋白相对分子质量80.37kD,理论等电点5.25。ThVP1编码蛋白的疏水性较强,含有13个跨膜区。氨基酸多序列比对结果显示,ThVP1具有典型的液泡膜H^＋-PPase家族3个高度保守片段（CS1、CS2和CS3）,与大豆VP1氨基酸序列一致性最高,为93%。系统进化分析表明,ThVP1属于I型液泡膜H^＋-PPase基因。实时荧光定量RT-PCR分析显示,NaCl和PEG胁迫下,ThVP1在柽柳根和叶中均呈现明显上调表达,表达量最高达到对照的20.9倍,暗示ThVP1可能在刚毛柽柳抗旱耐盐过程中发挥重要作用。

英文摘要：

A full length cDNA of a vacuolar H^＋-PPase gene（named ThVP1）was isolated from the transcriptome cDNA librarys of Tamarix hispida.ThVP1 was 3 022 bp in length,including an open reading frame of 2 298 bp which was predicted to encode a polypeptide of 765 amino acids.The estimated molecular weight and isoelectric points of the putative protein were 80.37 kD and 5.25,respectively.Hydrophobicity analysis and transmembrane domain prediction indicated that the ThVP1 contained 13potential transmembrane domains with strong hydrophobicity.The amino acids sequence of ThVP1 contains three conservative domains（CS1,CS2 and CS3）,which shows 93%identities in amino acids sequence to vacuolar H^＋-PPase genes fromReaumuria trigyna.Phylogenetic analysis indicates that ThVP1 belongs to class I type vacuolar H^＋-PPase gene.Quantitative real-time PCR assay revealed that the mRNA level of ThVP1 was significantly up-regulated by more than 20-fold higher than that of control under NaCl and PEG treatments in Tamarix hispida,suggesting that ThVP1 might play an important role in salt and drought tolerance of T.hispida.