中文摘要：

采用RNA加尾和引物延伸real time PCR法实时定量检测了黑腹果蝇Drosophila melanogaster的bantam、mir-14、mir-2a共3种microRNA（miRNA）在各发育阶段组织中的相对表达情况,发现3种miRNA在不同发育时期的表达差异明显,其表达变化规律与文献报道基本一致,验证了该法的可靠性.这种＂加尾和引物延伸＂方法,仅需1~10 ng总RNA或等同物,检测范围可达7个数量级,为昆虫miRNA研究提供了可靠的定量检测方法.

英文摘要：

The modified RNA-tailing and primer-extension qRT-PCR method was used to detect relative quantity of 3 microRNAs,bantam,mir-14 and mir-2a,in various developmental stages in Drosophila melanogaster.The results showed that the expression patterns of these miRNAs had the significant differences in different developmental stages,and the change dynamics of miRNAs relative quantities were consistent with the literatures which verified the reliability of this method.This method of RNA-tailing and primer-extension had advantages of high sensitivity,which only needed 1 to 10 ng total RNA or equivalents,and wide detection range with 107 grades,compared with other methods,which would provide a reliable method of quantification detection in insect miRNAs researches.