中文摘要：

目的：检测HepG2细胞中CYP3A、SLCO1B1、POR的单核苷酸多态性（SNP）位点CYP3A4＊1G、CYP3A4＊22、CYP3AP1＊3、CYP3A5＊3、SLCO1B1 T521C、SLCO1B1 A388G、POR＊28的基因型。方法：从HepG2细胞中提取基因组DNA,PCR扩增目的片段,对PCR扩增产物测序后分析上述7个多态性位点的基因型。结果与结论：CYP3A4＊1G、CYP3A4＊22、CYP3AP1＊3、CYP3A5＊3、SLCO1B1 T521C、SLCO1B1 A388G、POR＊28的基因型分别为GA、CC、GA、AG、TC、AG、CC。CYP3A4＊1G下游596 bp处基因型为CT,SLCO1B1 T521C下游50 bp和76 bp处基因型分别为TC和CT,POR＊28下游281 bp处基因型为GA。

英文摘要：

Aim： To detect the single nucleotide polymorphism（SNP）of drug metabolic enzyme CYP3A, transporter SLCO1B1 and cytochrome P450 oxidoreductase（POR）including CYP3A4＊1G, CYP3A4＊22, CYP3AP1＊3, CYP3A5＊3, SLCO1B1 T521C, SLCO1B1 A388G and POR＊28 in HepG2 cells.Methods： Genome DNA was obtained from HepG2 cells. Target gene segments were amplified by PCR. Then the genotype of the 7 alleles mentioned above were detected by sequencing the amplification products.Results and Conclusion： The genotypes of CYP3A4＊1G, CYP3A4＊22, CYP3AP1＊3, CYP3A5＊3, SLCO1B1 T521C, SLCO1B1 A388G, POR＊28 were GA, CC, GA, AG, TC, AG, and CC, respectively. Besides, the genotypes of SNP located 596 bp downstream of CYP3A4＊1G, those located 50 bp and 76 bp downstream of SLCO1B1 T521C, 281 bp downstream of POR＊28 were CT, TC, CT, and GA, respectively.