中文摘要：

目的：探讨生物学标记物对预测肝癌移植术后复发的价值。方法：回顾性分析2002年4月至2005年11月50例肝癌移植病例，存活者至少随访12月，石蜡切片做免疫组化检测E-cadherin、β-catenin、Ki-67的表达，分析其与复发、生存时间的相关性并与一些主要临床病理指标进行比较。结果：TNM分期、门静脉癌栓仅在单变量分析中对预测复发、生存时间有意义（P〈0.05），术前血清AFP仅在多变量分析中与复发有关（OR=2．552，P〈0．05）；胞膜E-cadherin低表达、核β-catenin阳性、核Ki-67高增殖指数在单变量分析中对预测复发、生存时间的统计学意义均显著，而且在多变量分析中结果类似（均P〈0．01），但胞膜β-catenin表达和预后无关（P〉0．05）。结论：这3个生物学标记物有前景成为肝癌移植术后复发的预后因子，且价值可能优于临床病理指标。

英文摘要：

AIM： To investigate the prognostic value of biomarkers on predicting recurrence of hepatocellular carcinoma （HCC） after orthotopic liver transplantation （OLT）. METHODS： Fifty patients with HCC who underwent OLT between April 2002 and November 2005 with a minimum clinical follow up of 12 months were included in this retrospective study. We examined immunohistochemical expression of E - cadherin with β - catenin, Ki - 67 proliferative index and analyzed the correlation between these biomarkers with recurrence and survival, along with the main clinical - pathological variables. RESULTS： Only at univariate analysis, TNM, portal vein tumor thrombi were valuable on predicting recurrence and survival time （ P 〈 0. 05 ）, and preoperative serum AFP correlate to recurrence only at multivariate analysis （ OR = 2. 552, P 〈 0. 05）. Lower membrane expression of E - cadherin, nuclear β - catenin localization and high Ki - 67 index showed notable significance on predicting recurrence and survival time at univariate analysis, as well at multivariate analysis, all P 〈 0. 01. The membrane expression of β- catenin did not correlate to the prognosis （ P 〉 0.05 ）. CONCLUSION： These three biomarkers might have potential as a tumor prognostic marker for predicting recurrence of HCC after OLT and perhaps are better than the clinical - pathological variables.