中文摘要：

目的：研究不同浓度内皮素-1（endothelin-1,ET-1）对牙周膜细胞（periodontal ligament cells,PDLCs）碱磷酶（alk aline ph osph atase,ALP）活性和骨钙素（osteocalcin,OCN）含量的影响。方法：体外培养牙周膜细胞,加入不同浓度（1,10,100n M）的ET-1,以未作任何处理的牙周膜细胞为对照组,刺激48h后观察ET-1对牙周膜细胞ALP活性和OCN含量的影响。结果：经ET-1（1,10,100n M）干预后,PDL细胞ALP活性和OCN含量低于对照组（P〈0.05）,并呈剂量依赖性。结论：ET-1对牙周膜细胞ALP活性和OCN含量具有抑制作用。

英文摘要：

Objective： To investigate how endothelin-1 affects the alkaline phosphatase（ ALP） activity and the production of osteocalcin（OCN） of periodontal ligament（PDL） cells. Methods： The PDL cells were stimulated with endothelin-1（1, 10, 100 n M） for 48 hours. The untreated h PDL cells served as normal control. ALP activity was analysed by colorimetric assay using ALP kit and the amount of OCN was assessed by osteocalcin ELISA kit. Results： The ALP activity and the production of OCN of PDL cells were reduced by ET-1 stimulation in dose-dependent manner. Conclusion： These results indicated that ET-1 could reduce the ALP activity and the production of OCN of PDL cells.