中文摘要：

非洲菊（Gerbera hybrida）是重要的园艺观赏花卉,也是研究复杂花序进化与发育的模式植物。花瓣的生长对园艺观赏花卉品质的形成具有重要贡献,阐明花瓣生长的分子机理将有助于增加对植物器官形态建成的认识,并为花卉品质的改良提供理论依据。为了在非洲菊中更好地开展基因功能研究,建立有效的转化系统十分必要。本研究以非洲菊舌状花花瓣为材料,通过农杆菌真空渗透法,分别以β-葡萄糖苷酸酶基因GUS和花色素苷合成关键转录因子基因GMYB10为报告基因,成功建立了花瓣瞬时表达及病毒诱导的基因沉默（virus-induced gene silence,VIGS）系统。该系统将为今后快速有效地开展非洲菊花瓣发育相关基因功能研究奠定技术基础。

英文摘要：

Gerbera hybrida is an important ornamental flower, and also an ideal model system for studying development and evolution mechanism of complex inflorescences. Petal growth is pivotal for ornamental flowers morphogenesis. Clarifying the genetic networks of petal morphogenesis will contribute to understand the organ- ogenesis as well as provide new theoretical basis for ornamental plant breeding. Thus, it is necessary to estab- lish an effective transformation system for in-depth identification of gene function in G. hybrida. In this study, fl-glucuronidase （GUS） and GMYBIO （key gene for anthocyanin biosynthesis in Gerbera petal） were selected as reporter genes to establish transient gene expression and virus-induced gene silencing （VIGS） system in Gerbera ray petals. Using Agrobacterium-mediated vacuum infiltration method, high-efficiency transformation systems hd been successfully established. This study will provide an effective tool for gene function analysis in G. hybrida petals.