为精确分析黄河三角洲刺槐根际与非根际土壤细菌群落定殖情况,本研究采用高通量测序方法对刺槐根际与非根际土壤细菌结构及多样性进行了研究。研究表明,根际土壤细菌共有36门214属,非根际土壤细菌共有33门153属。变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、酸杆菌门(Acidobacteria)细菌丰度超过15%,是根际与非根际土壤中的优势菌落。根际与非根际土壤中酸杆菌门、硝化螺旋菌门(Nitrospirae)丰度差异显著。根际土壤中红游动菌属(Rhodoplanes)、溶杆菌属(Lysobacter)、热单胞菌属(Thermomonas)、链霉菌属(Streptomyces)及非根际土壤中红游动菌属、溶杆菌属、链霉菌属、Kaistobacter细菌丰度超过4%。根际土壤中固氮菌丰度显著高于非根际土壤,解磷、解钾细菌丰度差异不显著。根际与非根际土壤细菌Chao丰富度分别为2054、2376,差异显著。根际与非根际土壤细菌之间的权重(Weighted Unifrac)距离在0.12~0.25之间。综上所述,黄河三角洲刺槐根际与非根际土壤细菌结构具有一定差异,多样性差异显著。
[Objective] As one of the three major pedogenic river deltas in China and the one that develops the fastest, the Yellow River Delta is an important reserved land resource. Developing mixed forests has been becoming a major strategy to develop the Yellow River Delta, because mixed forests can not only regulate temperature but also improve soil fertility. To improve the ecological system of the Yellow River Delta, a number of forest plantations have been established since the 1950s. Robinia pseudoacacia is the dominant tree species in most of the forest plantations, which contribute significantly to conserving soil and water and improving the ecological system of the Delta. Soil microbial community in plant rhizosphere plays an important role in plant nutrition and plant health. This research aimed to accurately evaluate how soil bacterial communities colonize in rhizosphere and in bulk soils under Robinia pseudoacacia in the Yellow River Delta. [Method] With the aid of the Illumina MiSeq technology, this research analyzed structure and diversity of the bacterial communities in the rhizosphere soil and the bulk soil under Robinia pseudoacacia for comparison. The differences in soil bacterial community between rhizosphere and bulk soil were expressed via the Weighted Unifrac Range of OTUs. [Result] Results show that the bacterial community in the rhizosphere soil consisted of 214 genera of 36 phyla and that in the bulk soil 153 genera of 33 phyla. Proteobacteria, Aetinobaeteria and Aeidobaeteria were the dominant phyla of bacteria in both rhizosphere and bulk soils with relative abundance being more than 15%. Acidobacteria and Nitrospirae varied sharply in relative abundance between the rhizosphere soil and the bulk soil. Chloroflexi, Gemmatimonadetes, Planetomycetes, Nitrospirae, and Verrucomicrobia were the dominant taxa of bacteria with relative abundance being more than 1%. Rhodoplanes, Lysobacter, Thermomonas and Streptomyces in the rhizosphere soil and Rhodoplanes,Lysobacter, Streptomyces and Kaistobacter in t