中文摘要：

以商业化的端羧基聚乳酸-羟基乙酸共聚物（PLGA-COOH）和氦基聚乙二醇羧基（NH_2-PEG-COOH）为原料,以生物型分子1-（3-二甲氦基丙基）-3-乙基碳二亚胺盐酸盐（EDC）和N-羟基琥珀酰亚胺（NHS）为媒介,以4-二甲氦基吡啶（DMAP）为催化剂,通过简单、绿色的羧基和胺基的偶联反应,制备了聚乳酸羟基乙酸/聚乙二醇（PLGA-PEG）共聚物分子,并以该PLGA-PEG分子为载体包裹色素上皮衍生因子（PEDF）,得到了PLGA-PEG/PEDF乳剂,并进一步考察了该乳剂作为抑制血管内皮生长因子（VEGF）的潜能.采用核磁共振氢谱表征了PLGA-PEG分子结构及其接枝率,并进一步表征了在不同条件下,PLGA-PEG/PEDF乳剂对人脐静脉内皮细胞（HUVEC）中VEGF水平的抑制作用.结果表明,以偶联法制备的PLGA-PEG分子为载体,得到的PLGA-PEG/PEDF乳剂具有良好的生物安全性,其对VEGF具有较高的抑制作用.

英文摘要：

A biodegradable poly（lactic-co-glycolic acid）/polyethylene glycol （PLGA-PEG） copolymer molecule was prepared by the simple and green coupling reaction between carboxyl group of poly（lactide-co-glycolide）-carboxylic acid endcap （PLGA-COOH） and amino group of poly（ethylene glycol）amine and carboxylic acid endcap （NH2-PEG-COOH） in the presence of 1-ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride （EDC） and N-hydroxysuccinimide （NHS）. The molecular structure of PLGA-PEG copolymer was characterized by 1H NMR spectroscopy, and the grafting yield of polyethylene glycol （PEG） on poly（lactic-co-glycolic acid） （PLGA） was calculated. Furthermore, pigment epithelium-derived factor （PEDF） was encapsulated in the PLGA-PEG copolymer to prepare a PLGA-PEG/PEDF emulsion for highly efficient inhibition on vascular endothelial growth gene expression （VEGF）. The results showed that the obtained PLGA-PEG copolymer was bio-safety and the PLGA-PEG/PEDF emulsion was a good agent for effective inhibition VEGF expression under different conditions. Thus, the PLGA-PEG copolymer can be used as a bio-carrier and the PLGA-PEG/PEDF emulsion can be used as a good angiogenesis inhibitor, which has a potential application for some diseases by anti angiogenesis.