目的:考察白术、茯苓多糖在胃肠道环境中的代谢情况。方法:白术、茯苓多糖分别与人工胃液、肠液和肠道菌共孵育,采用3,5-二硝基水杨酸法(DNS法)测定多糖的含量变化,考察白术、茯苓多糖在人工胃肠道环境中的代谢以及与肠道菌共孵育的变化。结果:白术、茯苓多糖在人工胃肠道环境中没有明显代谢,而与肠道菌的共孵育过程中则产生显著代谢。结论:白术、茯苓多糖在胃肠道中的代谢主要是在肠道中进行,肠道菌在此过程中发挥着重要作用。
Objective: To study the metabolism of Atractylodes Rhizome polysaccharides and pachymaran in gastrointestinal environments. Method: Atractylodes Rhizome polysaccharides and pachymaran were incubated with artificial gastric, intestinal juice and gut flora respectively; the method of 3,5-dinitrosalicylic acid (DNS) was applied to determine the variation of reducing sugar content, which suggested the metabolism of Atractylodes Rhizome polysaccharides and pachyman in gastrointestinal environments. Result: There was no significant metabolism found in artificial gastric juice and intestinal juice, while most of the polysaccharides were greatly reduced in the process of incubating with gut flora. Conclusion: The intestinal tract is the mainly metabolic place for Atractylodes Rhizome polysaccharides and pachyman, and the gut flora plays an important role in the process.