以本实验室保存的7种(13株)杜氏藻为材料,筛选出β-胡萝卜素含量较高的藻株Dunaliella.bardawil(1-7)进行紫外线诱变.以橙-红色藻落为初筛标志,再经高盐、强光、低氮诱导,检测其伊胡萝卜素含量,即复筛.发现突变株11-1具有高产β-萝卜素的能力,其β-胡萝卜索含量高达干质量的9.48%,显著高于原出发株.此外11-1的细胞体积是原出发株的5倍多,有利于养殖和收集.
The strains containing high content of β-carotene were obtained after the detection of 7 species ( 13 strains) of Dunaliella deposited in our laboratory. One of the strains was selected and radiated by UV. Experiment results demonstrated that the orange-red colonies of algae could be used as the preliminary marker for screening β-carotene-rich cell. A mutant, dominated as 11-1, containing β-carotene as high as 9.48% (dried mass) was selected after a serious of high salt concentration, high light intensity cultivation and lower nitrogen source conduction. Meanwhile the size of mutant 11-1 was 5 times more than its original pattern. It was propitious to cultivation and collection of the β-carotene hyper-producing new strains of Dunaliella.