中文摘要：

乳酸菌是一种公认的食品级微生物,它广泛存在于自然界中.很多编码其独特性状的基因位于游离于基因组DNA之外的质粒上.本文就乳酸菌质粒的提取方法、复制模式与特点以及改造和应用等方面进行概述,旨在揭示乳酸菌质粒的最新研究进展,为今后改造乳酸菌质粒,构建合适的基因工程载体提供参考与借鉴.

英文摘要：

Lactic acid bacteria are recognized food-grade microorganism, and widely exist in the nature. A lot of function genes are encoded by their plasmids, which are apart from genomic DNA. The extraction method of the plasmids from Lactic acid bacteria, replication patterns and characteristics as well as the modification and application, were reviewed in this paper. The aim of this paper is to reveal the latest research progress of the Lactic acid bacteria plasmids. And it provides a reference for the modification of the Lactic add bacteria plasmids as well s construct- ing suitable gene engineering vectors.