中文摘要：

常规皂化作用方法能导致更低的恢复和长链的丰满的酸的人工的变化。主要原因是在液体液体抽取过程期间在水、器官的层之间推迟的中间的层的错误判断。这研究证明中间的层由组成大量中等 -- 到为他们的特殊物理、化学的性质的长链的羧基的盐。一个改进皂化作用抽取方法也被开发，结果证明羧基的盐在中间的层散布了能完全被获得，它极大地提高丰满的酸分析的真实性和精确性。另外，中间的层的形成的可能的原因也被讨论。

英文摘要：

The conventional saponification method could result in lower recoveries and artificial changes of long- chain fatty acids. The main reason is the error judgment of the intermediate layer suspended between the aqueous and organic layer during the liquid-liquid extraction process. This study shows that the intermediate layer consists of lots of mediumto long-chain carboxylic salts for their special physical and chemical properties. An improved saponification extraction method is also developed and the results show that the carboxylic salts distributed in the interme- diate layer could be obtained completely, which greatly enhances the authenticity and accuracy of fatty acid anal- ysis. Additionally, the possible reasons of formation of the intermediate layer are also discussed.