中文摘要：

目的：探讨糖基化终末产物（AGEs-rtSA）对人牙周膜干细胞（HPDLSC）骨向分化能力的影响。方法：体外组织块法和有限稀释法克隆化培养牙周膜干细胞，流式细胞仪检测牙周膜细胞表型分子CD44、CDl46、stor-1、CD90，对其进行干细胞鉴定，将培养出的牙周膜干细胞与不同浓度的AGE-HSA共同培养，并矿化诱导后茜素红染色观察钙结节形成情况、碱性磷酸酶染色观察ALP活性、实时定量聚合酶链反应（realtimePCR）检测成骨基因表达情况。结果：流式细胞仪细胞表型分析CD44、CDl46、stor-1、CD90表达呈阳性。成骨诱导21d后茜素红染色，对照组和实验组均出现不同程度的矿化结节，定量分析显示1、10、100、200μg／mLAGEs组矿化能力均比对照组低，差异均有统计学意义（P〈0．05）；不同浓度的实验组之间矿化能力均有统计学差异（P〈0．05），且随着AGEs浓度的升高，矿化能力逐渐降低。成骨诱导7dAIJP染色比较发现各实验组均比对照组减弱。成骨诱导1周后RT-PCR检测各实验组的成骨基因ALP、Runx-2、Col-1、Runx-2mRNA表达水平均较对照组低，差异有统计学意义（P〈0．05）；不同浓度的实验组之间各成骨基因的表达也有统计学差异（P〈0．05）。结论：AGEs能抑制HPDLSC的骨向分化，并在一定范围内呈浓度依赖性。

英文摘要：

AIM： To investigate the effect of advanced glycation end products （AGEs） on the osteogenic differentiation of human periodontal ligament stem cells （HPDLSCs）. METHODS ： PDLSCs were isolated by limited dilution of culture ceils for single cell clone. Flow cytometry was employed to study the surface marker of the cloned cells. Passage 3 PDLSCs were induced with different concentrations of AGEs. The osteogenic differentiation capacity of hPDLSCs was evaluated by Alizarin red staining, ALP and real-time quantitative reverse transcription polymerase chain reaction （realtion RT-PCR）. RESULTS： Expression of cell surface molecules CD44, CD146, stor-1 and CD90 of AGEs - induced group was significantly higher than that of the control group by flow cytometry. After 21 -day induc- tion, Alizarin red staining showed varying degree of mineralization nodules, the expressions of osteogenic genes （ALP,Runx-2, Co1-1 ） of unstimulated group were significantly higher than those of AGEs-PDLSCs group by real time PCR after 7-day induction （P〈0.05）. CONCLUSION：AGEs reduce the osteogenic differentiation capacity of PDLSCs in a concentration-dependent manner.