中文摘要：

背景：神经生长因子和髓磷脂相关糖蛋白能够促进损伤神经的结构与功能恢复,但目前未见通过转基因技术增加受损周围神经中神经生长因子与髓磷脂相关糖蛋白基因表达的报道。目的：探讨神经生长因子和髓磷脂相关糖蛋白双基因经腺病毒介导在大鼠损伤坐骨神经的共表达效果。方法：将40只SD大鼠随机分为正常对照组、坐骨神经损伤组、空病毒组、神经生长因子组和神经生长因子/髓磷脂相关糖蛋白组。后4组切断并缝合右侧坐骨神经建立坐骨神经损伤模型后,分别肌肉注射生理盐水、生理盐水、空腺病毒（1×108 PFU/次）、携带神经生长因子的腺病毒（1×108 PFU/次）和携带神经生长因子和髓磷脂相关糖蛋白的腺病毒（1×108 PFU/次）,每2 d注射1次,连续注射3次。结果与结论：神经生长因子组和神经生长因子/髓磷脂相关糖蛋白组大鼠坐骨神经中神经生长因子表达水平显著高于空病毒组和正常对照组（P〈0.05）;神经生长因子/髓磷脂相关糖蛋白组大鼠坐骨神经中髓磷脂相关糖蛋白表达水平显著高于空病毒组、神经生长因子组和正常对照组（P〈0.05）。提示腺病毒介导的神经生长因子/髓磷脂相关糖蛋白双基因可有效表达于大鼠损伤坐骨神经。

英文摘要：

BACKGROUND： Nerve growth factor（NGF） and myelin-associated glycoprotein（MAG） can promote the repair of peripheral nerve. However, increasing the expression of NGF and MAG in the injured peripheral nerve by transgene technology has not been reported. OBJECTIVE： To investigate the expression of NGF and MAG after adenovirus（Ad） mediated transfection in the injured sciatic nerve in rats. METHODS： Forty Sprague-Dawley rats were randomly divided into normal, model, Ad, NGF and NGF-MAG groups. Sciatic nerve injury models were established in the latter four groups by cutting and suturing the right sciatic nerve. Afterwards, the rats in each group were subject to the intramuscular injection of normal saline, normal saline, Ad（1×10-8 PFU）, Ad-NGF（1×10-8 PFU）, and Ad-NGF-MAG（1×10-8 PFU）, respectively, once every 2 days, for consecutive three times. RESULTS AND CONCLUSION： The expression level of NGF in the rat sciatic nerve in the Ad and normal groups was significantly higher than that in the NGF and NGF-MAG groups（P〈0.05）. Compared with the Ad, NGF and normal groups, the expression level of MAG in the NGF-MAG group was significantly increased（P〈0.05）. To conclude, the Ad-mediated NGF and MAG after double gene transfection were expressed effectively in the injured sciatic nerve in rats.