中文摘要：

目的探讨人参皂甙Rg1对软骨细胞凋亡的影响。方法正常大鼠关节软骨细胞分为三组：A、B组用白细胞介素18（IL-113）10ng／ml作用24h，建立软骨细胞凋亡模型；B组事先加入10μg／ml的人参皂甙Rg1保护2h；C组作为空白对照。TUNEL法检测人参皂甙Rg1对软骨细胞凋亡的影响；Westernblot检测人参皂甙Rg1对凋亡软骨细胞基质金属蛋白酶13（MMP-13）和基质金属蛋白酶组织抑制剂1（TIMP-1）蛋白表达的影响。结果A、B和C组细胞凋亡率分别为（37．30±4．05）％、（10．30±1．08）％和（2．20±1．93）％，B组胞凋亡率低于A组（P〈0．05）。与A组比较，B组软骨细胞TIMP-1的表达增加，MMP-13的表达降低（P〈0．05）。结论人参皂甙Rg1具有拮抗大鼠关节软骨细胞凋亡的作用。

英文摘要：

Objective To investigate the influence of Ginsenoside Rgl against interleukin-1 beta （IL-1β） - induced apoptosis in rat articular chondrocytes. Methods The articular chondrocytes were divided into three groups. The ehondrocytes in groups of A and B were exposed to IL-1β 10 ng/ml for 24 h to create apoptosis model. Ginsenoside Rgl 10 μg/ml was added at 2 h before IL-1β administration in group B. Group C was taken as blank control. The chondrocyte apoptosis was assessed by TUNEL staining and the expressions of MMP-13 and TIMP-i were detected by Western blot. Results The chondrocyte apoptosis rates in groups of A,B and C were （37. 30±4.05）%, （10. 30±1.08）% and （2.20± 1.93）%, respectively, which was lower in group B than that in group A（P〈0. 05）. Compared to group A, the expression of TIMP-1 was higher, but that of MMP-13 was lower, in group B （P〈0. 05）. Conclusion Ginsenoside Rgl can protect articular chondrocytes from apoptosis induced by IL-1β in rats.