中文摘要：

目的构建并鉴定细粒棘球绦虫（Eg）转基因植物载体重组pBI—Eg95质粒。方法从细粒棘球蚴包囊中分离原头节，经超声粉碎后抽提总RNA，反转录成cDNA，设计合成引物，以cDNA为模板，通过PCR从cDNA中扩增出目的基因Eg95，经电泳及测序鉴定后。将该基因定向克隆到植物表达载体pBI121中构建pBI—Eg95重组质粒，电穿孔转化根癌农杆菌（At）LBA4404株；从转化的At阳性株中抽提质粒进行双酶切和以抽提的质粒为模板进行PCR鉴定。结果RT—PCR扩增出1条约471bp的特异性条带．DNA序列分析与GenBank已知的序列同源性为100％，从转化的At中抽提的质粒，双酶切及PCR测定的结果与预期相符。结论成功构建了细粒棘球绦虫转基因植物载体重组pBI—Eg95质粒，为进一步构建细粒棘球绦虫转基因植物疫苗奠定了基础。

英文摘要：

Objective To construct and identify the transgenic plant vector recombinant pBI-Eg95 plasmid of Echinococcus granulosus. Methods Total RNA was extracted from hydatid cyst protoscoleces of Echinococcus granulosus after sonication. A couple of specific primers were designed on the basis of known sequences of Eg95 gene. The desired gene was amplified by PCR technique from the cDNA, and then was cloned into the plant expression vector pBI121 to construct the recombinant pBI-Eg95 plasmid. The recombinant plasmid was electroporated into Agrobacterium tumefaciens （At） LBA4404 strain. The positive recombinant clones were confirmed by restriction endonuclease digestion and characterized by PCR. Results For RT-PCR, a specific band around 471 bp was amplified. The result of DNA sequencing of Eg95 showed the identity with the published sequence. The same band was obtained by restriction endonuclease digestion and PCR from the plasmids of positive recombinant At（rAt）. Conclusions The recombinant pBI-Eg95 plasmid was successfully constructed, and it provides the basis to further research of the transgenic plant vaccine of Echinococcus granulosus.