采用醋酸钠.抗生素分离法对超市牛奶样品进行苏云金芽胞杆菌(Bacillus thurgngiensis,Bt)的分离,获得2株Bt菌株,分别命名为BtBRC—LJ1和BtBRC-LJ2。利用PCR方法对Bt BRC-LJ1和BtB RC-LJ2菌株幼皿和nheC肠毒素基因的分布进行检测。结果显示,这2个菌株均含有这两种肠毒素基因。
Milk products were collected from supermarkets in Fuzhou, Fujian Province to isolate Bacillus thuringiensis(Bt) strains by the method of sodium acetate adding antibiotic. And two Bt strains designated as Bt BRC-LJI and Bt BRC-LJ2 were isolated. The presence of hblD and nheC enterotoxin genes from these two Bt strains were detected respectively by PCR. The results showed that two Bt strains both contained hb/D and nheC genes.